Document Detail


Comparison of p53 and Epidermal Growth Factor Receptor Gene Status Between Primary Tumors and Lymph Node Metastases in Non-Small Cell Lung Cancers.
MedLine Citation:
PMID:  20811949     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND: To obtain insight into the cancer progression and metastatic process, we evaluate p53/epidermal growth factor receptor (EGFR) somatic aberrations in non-small-cell lung cancers to compare accumulated genetic alterations between primary tumors and lymph node metastases.
MATERIALS AND METHODS: A total of 56 primary lung cancers with corresponding lymph node metastases were identified to investigate somatic mutations and altered expressions of p53 and EGFR for clonality assessment. Genomic DNA was extracted from macrodissected cells of paraffin-embedded primary tumor and metastatic lymph node tissues. Overexpression and somatic mutations in exons of p53 (exons 5-8) and tyrosine kinase domain of EGFR (exons 18-21) were examined by immunohistochemical staining and DNA sequencing, respectively.
RESULTS: p53 and EGFR mutation/overexpression status were different between primary tumors and lymph node metastases in 5.4/7.2% and 28.6/33.9%, respectively. In most cases, the p53 and EGFR mutations usually preceded lymph node metastasis, and these gene statuses in the primary cancer and their lymph node metastasis were concordant (92.9 and 69.6%, respectively), which further supported the hypothesis that when these p53 mutations occur before the establishment of lymph node metastasis, they subsequently persist in the metastatic nodes. The expressions of p53 and EGFR showed 7.1 and 33.9% discordance in that order.
CONCLUSIONS: Our results reveal that p53 and EGFR mutations usually precede lymph node metastasis. The higher prevalence of EGFR heterogeneity existing in the primary tumor is not reflected in all lymph node metastasis and thus might have therapeutic implications when adjuvant therapy is considered.
Authors:
Yih-Leong Chang; Chen-Tu Wu; Jin-Yuan Shih; Yung-Chie Lee
Related Documents :
4014539 - Epithelioid sarcoma. diagnosis, prognostic indicators, and treatment.
6367969 - Lymphatic metastasis.
2682099 - Relation of lymph-node metastasis to histopathologic appearance in oral cavity and orop...
21687669 - Aerosolized bc-819 inhibits primary but not secondary lung cancer growth.
20616339 - Tumor lymphangiogenesis and metastasis to lymph nodes induced by cancer cell expression...
8878019 - Hyperplastic mesothelial cells within abdominal lymph nodes: mimic of metastatic ovaria...
16525659 - Corona enhancement in ultrasonographical post-vascular phase images with microbubble co...
23977139 - Down-regulation of c9orf86 in human breast cancer cells inhibits cell proliferation, in...
3464799 - Tumor progression in nude mice and its representation in cell culture.
Publication Detail:
Type:  Journal Article     Date:  2010-09-02
Journal Detail:
Title:  Annals of surgical oncology     Volume:  18     ISSN:  1534-4681     ISO Abbreviation:  Ann. Surg. Oncol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9420840     Medline TA:  Ann Surg Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  543-50     Citation Subset:  IM    
Affiliation:
Department of Pathology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Tyrosine Kinase Inhibitor PTK/ZK Enhances the Antitumor Effects of Interferon-?/5-Fluorouracil Thera...
Next Document:  Lipid metabolism in long-lived families: the Leiden Longevity Study.