Document Detail

Comparison of orthogonal liquid and gas chromatography-mass spectrometry platforms for the determination of amino acid concentrations in human plasma.
MedLine Citation:
PMID:  20696434     Owner:  NLM     Status:  MEDLINE    
Concentrations of amino acids in a human plasma pool were determined using four independent quantification methods. Orthogonal separation schemes (LC, GC, or GC x GC) and detection systems (triple quadrupole or time-of-flight mass spectrometry) are shown to demonstrate excellent consistency among platforms for quantifying 18 amino acids in NIST Standard Reference Material (SRM) 1950 Metabolites in Human Plasma using a well-characterized isotope dilution (ID) quantification method. Measured levels were consistent with reference values in plasma from the literature. Individual amino acid concentrations in plasma varied by over an order of magnitude ranging from 1.83 microg/g to 28.0 microg/g (7.78 micromol/L to 321 micromol/L). Average variability (coefficient of variation) between experimental amino acid concentrations (excluding cysteine) among all methods was 6.3%. Certified mass fraction values for amino acids in NIST SRM 1950 will be established from statistically weighted means of all experimental results.
Elizabeth A McGaw; Karen W Phinney; Mark S Lowenthal
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2010-07-21
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1217     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-23     Completed Date:  2010-12-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  5822-31     Citation Subset:  IM    
Copyright Information:
Published by Elsevier B.V.
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20988-8392, USA.
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MeSH Terms
Amino Acids / blood*
Chromatography, Liquid / methods*
Gas Chromatography-Mass Spectrometry / methods*
Isotope Labeling
Linear Models
Reproducibility of Results
Sensitivity and Specificity
Reg. No./Substance:
0/Amino Acids

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