| Comparison of a novel HPV test with the Hybrid Capture II (hcII) and a reference PCR method shows high specificity and positive predictive value for 13 high-risk human papillomavirus infections. | |
| | |
MedLine Citation:
|
PMID: 18262463 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: It is well established that human papillomavirus (HPV) infection is highly related to the development of precursor lesions of cervical cancer and uterine cancers. However, for a pre-cancerous lesion to develop, a persistent infection with a high-risk type HPV is necessary. The Digene Hybrid Capture II (hcII) assay is the only FDA approved method used in conjunction with cytology for HPV screening of women older than 30. The hcII has moderate sensitivity (64.7%) and is dependent on the cellular content of samples, rendering occasionally false positive and false negative results. OBJECTIVE: This study aims to evaluate the performance of a new HPV diagnostic kit (High-Risk HPV detection kit, manufactured by Human Genetic Signatures (HGS), Sydney, Australia). METHODS: The method under evaluation was assessed by comparing the results obtained from testing 834 cervical specimens with the HGS method and the Digene hcII method, using genotyping as the reference standard. RESULTS: Results of the study showed that the specificity and positive predictive value of the HGS High-Risk HPV detection test are significantly greater than those of the Digene hcII test. Overall the HGS HPV assay provides a more accurate system for the detection of high-risk HPV strains, with simpler technical use compared with PCR-sequencing methods. |
| | |
Authors:
|
Cristina Baleriola; Douglas Millar; John Melki; Neralie Coulston; Phillip Altman; Nikolas Rismanto; William Rawlinson |
Related Documents
:
|
17392713 - Advances in primary and secondary interventions for cervical cancer: human papillomavir... 21072553 - Systemic lupus erythematosus, human papillomavirus infection, cervical pre-malignant an... 14965333 - A mechanistic overview on male infertility and germ cell cancers. |
Publication Detail:
|
Type: Comparative Study; Evaluation Studies; Journal Article Date: 2008-02-08 |
Journal Detail:
|
Title: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology Volume: 42 ISSN: 1386-6532 ISO Abbreviation: J. Clin. Virol. Publication Date: 2008 May |
Date Detail:
|
Created Date: 2008-04-29 Completed Date: 2008-07-24 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9815671 Medline TA: J Clin Virol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 22-6 Citation Subset: IM |
Affiliation:
|
Virology Division, Department of Microbiology, South Eastern Area Laboratory Services, Prince of Wales Hospital, Randwick, NSW 2031, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Australia Female Humans Nucleic Acid Hybridization / methods Papillomaviridae / classification*, isolation & purification* Papillomavirus Infections / diagnosis*, virology* Polymerase Chain Reaction / methods Predictive Value of Tests Reagent Kits, Diagnostic Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Reagent Kits, Diagnostic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Study on the interaction of puerarin with lysozyme by spectroscopic methods.
Next Document: Mercury in breast milk - a health hazard for infants in gold mining areas?