Document Detail


Comparison of a new synthetic, peptide-derived, polyclonal antibody-based, immunohistochemical test with in situ hybridisation for the detection of swine hepatitis E virus in formalin-fixed, paraffin-embedded tissues.
MedLine Citation:
PMID:  18701328     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A synthetic, peptide-derived, polyclonal antibody-based, immunohistochemical test was developed to detect swine hepatitis E virus (HEV) and was compared with in situ hybridisation for the detection of HEV in formalin-fixed, paraffin-embedded tissues from experimentally infected pigs. Solid-phase peptide synthesis was used to generate peptides from swine HEV open reading frame 2, and the purified peptides were injected into rabbits to produce polyclonal antibodies. The specificity and sensitivity of the test were both 100%. Liver was most consistently positive for swine HEV antigen and RNA by immunohistochemistry and in situ hybridisation, respectively, but both were detected much less frequently in extrahepatic tissues such as lymph node, tonsil, spleen, and intestine. Swine HEV antigen and RNA showed a similar distribution in virus-infected hepatocytes in serial sections. The novel test developed in this study is suitable for consistently detecting swine HEV antigen in formalin-fixed, paraffin-embedded tissues.
Authors:
Y-H Lee; Y Ha; K-K Ahn; K-D Cho; B H Lee; S-H Kim; C Chae
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-12
Journal Detail:
Title:  Veterinary journal (London, England : 1997)     Volume:  182     ISSN:  1532-2971     ISO Abbreviation:  Vet. J.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-06-15     Completed Date:  2009-07-22     Revised Date:  2009-08-20    
Medline Journal Info:
Nlm Unique ID:  9706281     Medline TA:  Vet J     Country:  England    
Other Details:
Languages:  eng     Pagination:  131-5     Citation Subset:  IM    
Affiliation:
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, San 56-1, Shillim-dong, Gwanak-gu, Seoul 151-742, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Viral / analysis
Antigens, Viral / immunology*
Hepatitis E / immunology,  pathology,  veterinary*,  virology
Hepatitis E virus / genetics,  immunology*,  isolation & purification
Immunohistochemistry / methods,  standards,  veterinary*
In Situ Hybridization / veterinary
Liver / pathology
Organ Specificity
Peptides / chemical synthesis,  immunology
RNA, Viral / analysis
Sensitivity and Specificity
Swine
Swine Diseases / immunology,  pathology*,  virology
Viral Proteins / analysis*,  immunology
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Antigens, Viral; 0/Peptides; 0/RNA, Viral; 0/Viral Proteins

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