Document Detail


Comparison of a new anti-glutamic acid decarboxylase (GAD) enzyme-linked immunosorbent assay (ELISA) with radioimmunoassay methods: a multicenter study.
MedLine Citation:
PMID:  9288579     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several methods are available for the measurement of antibodies to glutamic acid decarboxylase (anti GAD). These antibodies are valuable tools for the immunodiagnosis of insulin-dependent (type 1) diabetes mellitus (IDDM) and for the assessment of risk for the future development of IDDM. We here describe a new enzyme-linked immunosorbent assay (ELISA) for the detection of anti-GAD which was tested in a multicenter study. The results of the new anti-GAD ELISA correlate well with those obtained by radioimmunoassays (RIA) and they have a higher sensitivity (69%) and specificity (98%) compared to other anti-GAD enzyme immunoassays as determined in the IDW Proficiency Test Program for the detection of GAD antibodies. The new ELISA is simple and easy to perform, with convenient handling of the reagents. Quantitative and reproducible test results are available within approximately four hours. The new anti-GAD ELISA can be used for large scale population screening to indicate a prediabetic state as well as to diagnose autoimmune diabetes in adults (LADA) and the risk for IDDM in pregnant women with gestational diabetes.
Authors:
T Wild; W A Scherbaum; H Gleichmann; M Landt; J Santiago; J Endl; R Landgraf; M G Cavallo; M Ganz; P Pozzilli
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Publication Detail:
Type:  Comparative Study; Journal Article; Multicenter Study    
Journal Detail:
Title:  Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme     Volume:  29     ISSN:  0018-5043     ISO Abbreviation:  Horm. Metab. Res.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-10-24     Completed Date:  1997-10-24     Revised Date:  2009-02-19    
Medline Journal Info:
Nlm Unique ID:  0177722     Medline TA:  Horm Metab Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  403-6     Citation Subset:  IM    
Affiliation:
Boehringer Mannheim GmbH, Tutzing/Mannheim, Germany. Thomas_Wild@bmg.boehringer-mannheim.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Autoantibodies / blood*
Autoimmune Diseases / diagnosis,  immunology
Diabetes Mellitus, Type 1 / diagnosis*,  immunology
Enzyme-Linked Immunosorbent Assay*
Female
Glutamate Decarboxylase / immunology*
Humans
Male
Prediabetic State / diagnosis,  immunology
Predictive Value of Tests
Pregnancy
Pregnancy in Diabetics / diagnosis,  immunology
Radioimmunoassay*
Chemical
Reg. No./Substance:
0/Autoantibodies; EC 4.1.1.15/Glutamate Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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