| Comparison of murine fibroblast cell response to fluor-hydroxyapatite composite, fluorapatite and hydroxyapatite by eluate assay. | |
| | |
MedLine Citation:
|
PMID: 19378927 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Fluorapatite (FA) is one of the inorganic constituents of bone or teeth used for hard tissue repairs and replacements. Fluor-hydroxyapatite (FHA) is a new synthetic composite that contains the same molecular concentration of OH(-) groups and F(-) ions. The aim of this experiment was to evaluate the cellular responses of murine fibroblast NIH-3T3 cells in vitro to solid solutions of FHA and FA and to compare them with the effect of hydroxyapatite (HA). We studied 24, 48 and 72 h effects of biomaterials on cell morphology, proliferation and cell cycle of NIH-3T3 cells by eluate assay. Furthermore, we examined the ability of FHA, FA and HA to induce cell death and DNA damage. Our cytotoxic/antiproliferative studies indicated that any of tested biomaterials did not cause the total inhibition of cell division. Biomaterials induced different antiproliferative effects increasing in the order HA < FHA < FA which were time- and concentration-dependent. None of the tested biomaterials induced necrotic/apoptotic death of NIH-3T3 cells. On the other hand, after 72 h we found that FHA and FA induced G0/G1 arrest of NIH-3T3 cells, while HA did not affect any cell cycle phases. Comet assay showed that while HA demonstrated weaker genotoxicity, DNA damage induced by FHA and FA caused G0/G1 arrest of NIH-3T3 cells. Fluoridation of hydroxyapatite and different FHA and FA structure caused different cell response of NIH-3T3 cells to biomaterials. |
| | |
Authors:
|
Sona Jantová; Silvia Letasiová; Marica Theiszová; M Palou |
Related Documents
:
|
10816327 - Apoptotic and non-apoptotic modes of programmed cell death in mcf-7 human breast carcin... 11916077 - Procoagulant activity of t lymphoblastoid cells due to exposure of negatively charged p... 20622327 - Effect of autophagy on paclitaxel-induced caski cell death. 14634337 - Role of ca2+, mg2+ and k+ ions in determining apoptosis and extent of suppression affor... 2820507 - Intracellular chromium reduction. 9653147 - Mutagenicity of arsenic in mammalian cells: role of reactive oxygen species. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Acta biologica Hungarica Volume: 60 ISSN: 0236-5383 ISO Abbreviation: Acta. Biol. Hung. Publication Date: 2009 Mar |
Date Detail:
|
Created Date: 2009-04-21 Completed Date: 2009-05-14 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8404358 Medline TA: Acta Biol Hung Country: Hungary |
Other Details:
|
Languages: eng Pagination: 89-107 Citation Subset: IM |
Affiliation:
|
Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK-81237 Bratislava, Slovak Republic. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apatites / pharmacology* Cell Cycle / drug effects* Cell Death / drug effects Cell Proliferation / drug effects* Cell Shape / drug effects* DNA Damage / drug effects Hydroxyapatites / pharmacology* Mice NIH 3T3 Cells |
| Chemical | |
Reg. No./Substance:
|
0/Apatites; 0/Hydroxyapatites; 1306-05-4/fluorapatite; 55575-17-2/fluor-hydroxylapatite |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Changes in antioxidant status and biochemical parameters after orally cadmium administration in fema...
Next Document: A comparative analysis of fatty acid composition of root and shoot lipids in Zea mays under copper a...