| Comparison of midgut bacterial diversity in tropical caterpillars (lepidoptera: saturniidae) fed on different diets. | |
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MedLine Citation:
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PMID: 22251723 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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As primary consumers of foliage, caterpillars play essential roles in shaping the trophic structure of tropical forests. The caterpillar midgut is specialized in plant tissue processing; its pH is exceptionally alkaline and contains high concentrations of toxic compounds derived from the ingested plant material (secondary compounds or allelochemicals) and from the insect itself. The midgut, therefore, represents an extreme environment for microbial life. Isolates from different bacterial taxa have been recovered from caterpillar midguts, but little is known about the impact of these microorganisms on caterpillar biology. Our long-term goals are to identify midgut symbionts and to investigate their functions. As a first step, different diet formulations were evaluated for rearing two species of tropical saturniid caterpillars. Using the polymerase chain reaction (PCR) with primers hybridizing broadly to sequences from the bacterial domain, 16S rRNA gene libraries were constructed with midgut DNA extracted from caterpillars reared on different diets. Amplified rDNA restriction analysis indicated that bacterial sequences recovered from the midguts of caterpillars fed on foliage were more diverse than those from caterpillars fed on artificial diet. Sequences related to Methylobacterium sp., Bradyrhizobium sp., and Propionibacterium sp. were detected in all caterpillar libraries regardless of diet, but were not detected in a library constructed from the diet itself. Furthermore, libraries constructed with DNA recovered from surface-sterilized eggs indicated potential for vertical transmission of midgut symbionts. Taken together, these results suggest that microorganisms associated with the tropical caterpillar midgut may engage in symbiotic interactions with these ecologically important insects. |
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Authors:
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Adrián A Pinto-Tomás; Ana Sittenfeld; Lorena Uribe-Lorío; Felipe Chavarría; Marielos Mora; Daniel H Janzen; Robert M Goodman; Holly M Simon |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Environmental entomology Volume: 40 ISSN: 1938-2936 ISO Abbreviation: Environ. Entomol. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2012-01-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502320 Medline TA: Environ Entomol Country: United States |
Other Details:
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Languages: eng Pagination: 1111-22 Citation Subset: IM |
Affiliation:
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Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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