Document Detail

Comparison of the microtitration agglutination test and the enzyme-linked immunosorbent assay for the detection of herds affected with swine dysentery.
MedLine Citation:
PMID:  6349439     Owner:  NLM     Status:  MEDLINE    
A method was designed to evaluate and compare the microtitration agglutination test (MAT) and the enzyme-linked immunosorbent assay (ELISA) to detect antibodies in swine sera to Treponema hyodysenteriae and thereby establish a method for determining the prevalence of swine dysentery (SD) in herds. According to sampling criteria based on the hypergeometric distribution, sera were collected from 3 age groups of swine from farms having a history of SD on the premises (SD+) recently or being free of the disease (SD-). The highest degree of test sensitivity was obtained when sera from market age swine were evaluated with the ELISA. Of 14 SD+ herds from which sera were obtained from market-age swine, 13 were positive with the ELISA (93%); none of the 8 SD- herds was positive. The detection rates of individual swine in the SD+ herds for the 2 tests by age group were as follows: MAT--adult swine 1.4%, market-age swine 6%, and weaned pigs 0.8%; ELISA--adult swine 16%, market swine 31%, and weaned pigs 0.5%.
I T Egan; D L Harris; L A Joens
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  American journal of veterinary research     Volume:  44     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  1983 Jul 
Date Detail:
Created Date:  1983-09-23     Completed Date:  1983-09-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1323-8     Citation Subset:  IM    
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MeSH Terms
Age Factors
Agglutination Tests / veterinary*
Antibodies, Bacterial / analysis
Dysentery / diagnosis,  veterinary*
Enzyme-Linked Immunosorbent Assay*
Immunoenzyme Techniques*
Swine Diseases / diagnosis*
Treponema / immunology
Treponemal Infections / diagnosis,  veterinary*
Reg. No./Substance:
0/Antibodies, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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