Document Detail


Comparison of microsatellite and antigen-coding loci for differentiating recrudescing Plasmodium falciparum infections from reinfections in Kenya.
MedLine Citation:
PMID:  16442537     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have compared the ability of five Plasmodium falciparum microsatellites and three antigen-coding loci to differentiate recrudescence from reinfection. We used 133 pairs of P. falciparum-infected blood samples collected during in vivo drug efficacy trials from three sites in Kenya with different malaria endemicities. There were no significant differences between the marker subsets in their ability to discriminate recrudescences from new infections across the three sites. Overall, microsatellite loci revealed significantly higher expected heterozygosity and multiplicity of infection levels than antigen-coding loci. The mean expected heterozygosity across all loci in the three populations was significantly higher with microsatellites (0.70, 0.78 and 0.79) than antigen-coding loci (0.53, 0.60 and 0.62) for Mwea, Tiwi and Bondo areas, respectively. These observations can be explained by three non-exclusive hypotheses: (i) microsatellites are more polymorphic than antigenic loci; (ii) partially immune hosts remove certain parasites from infections on the basis of their antigenic alleles; and/or (iii) recombination occurs in vitro or in vivo with microsatellites.
Authors:
Jonathan M Mwangi; Sabah A Omar; Lisa C Ranford-Cartwright
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-11-28
Journal Detail:
Title:  International journal for parasitology     Volume:  36     ISSN:  0020-7519     ISO Abbreviation:  Int. J. Parasitol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-17     Completed Date:  2006-11-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0314024     Medline TA:  Int J Parasitol     Country:  England    
Other Details:
Languages:  eng     Pagination:  329-36     Citation Subset:  IM    
Affiliation:
Kenya Medical Research Institute, Centre for Biotechnology Research and Development, Mbagathi Road, P.O. Box 54840-00200, Nairobi. Kenya.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Protozoan / genetics*
Antimalarials / therapeutic use
DNA, Protozoan / genetics
Drug Resistance
Endemic Diseases
Genetic Markers / genetics
Heterozygote
Humans
Kenya / epidemiology
Malaria, Falciparum / drug therapy,  epidemiology,  genetics*
Microsatellite Repeats / genetics*
Polymerase Chain Reaction / methods
Polymorphism, Genetic / genetics
Recurrence
Chemical
Reg. No./Substance:
0/Antigens, Protozoan; 0/Antimalarials; 0/DNA, Protozoan; 0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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