| Comparison of mice born after intracytoplasmic sperm injection with in vitro fertilization and natural mating. | |
| | |
MedLine Citation:
|
PMID: 16998805 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The procedures of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are routinely used in modern medicine to overcome infertility and, in animal husbandry, to propagate lines with compromised fertility. However, there remains concern that manual selection and injection of whole sperm into oocytes could contribute to pre- and postnatal developmental defects. To address this, we have used gene expression profiling and immunophenotyping to characterize offspring generated by these procedures. We used gametes from glutathione peroxidase 1 knockout (Gpx1-/-) mice as a sensitized screen responsive to oxidative stress from artificial reproduction technologies (ART). There were no differences between IVF and ICSI derived offspring in gene expression patterns, and minor differences in hematopoietic parameters. Furthermore there were only minor differences between these IVF and ICSI pups and those derived from natural mating. These data demonstrate for the first time in that there is no significant phenotypic affects of ICSI when compared to IVF and we identified a relatively minor influence of the artificial fertilization methods on phenotype of offspring compared with natural mating. These observations would support the use of ICSI for derivation of mutant mouse lines and may be of some importance for the use of this technique in human ART. |
| | |
Authors:
|
Trevor J Wilson; Orly Lacham-Kaplan; Jodee Gould; Andrew Holloway; Ivan Bertoncello; Paul J Hertzog; Alan Trounson |
Related Documents
:
|
23482325 - Lin28b promotes head and neck cancer progression via modulation of the insulin-like gro... 23073835 - The rna pol ii sub-complex hsrpb4/7 is required for viability of multiple human cell li... 2828165 - Cis- and trans-acting sequences required for expression of simian virus 40 genes in mou... 20130115 - Hydroxysteroid (17{beta}) dehydrogenase 12 is essential for mouse organogenesis and emb... 17167715 - Treatment of human tumor cells by combine gene therapy harnessing plasmids expressing h... 10933955 - Optimization of nonviral gene transfer of vascular smooth muscle cells in vitro and in ... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Molecular reproduction and development Volume: 74 ISSN: 1040-452X ISO Abbreviation: Mol. Reprod. Dev. Publication Date: 2007 Apr |
Date Detail:
|
Created Date: 2007-01-31 Completed Date: 2007-03-28 Revised Date: 2007-12-03 |
Medline Journal Info:
|
Nlm Unique ID: 8903333 Medline TA: Mol Reprod Dev Country: United States |
Other Details:
|
Languages: eng Pagination: 512-9 Citation Subset: IM |
Affiliation:
|
Centre for Functional Genomics and Human Disease, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn* / immunology, metabolism Female Fertilization / physiology* Fertilization in Vitro / veterinary Gene Expression Profiling Glutathione Peroxidase / genetics Immunophenotyping Male Mice Mice, Inbred C57BL Mice, Inbred CBA Mice, Knockout Pregnancy Pregnancy, Animal Sperm Injections, Intracytoplasmic / veterinary* |
| Grant Support | |
ID/Acronym/Agency:
|
U01 HD38228/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
EC 1.11.1.-/glutathione peroxidase GPX1; EC 1.11.1.9/Glutathione Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: ERK1/2 regulates two sequential steps promoting monocyte survival to peroxynitrite.
Next Document: Absence of Bcl-2 expression favors response to the short-term administration of diethylstilbestrol d...