Document Detail


Comparison of the metabolic activities of four wild-type Clostridium perfringens strains with their gatifloxacin-selected resistant mutants.
MedLine Citation:
PMID:  19855959     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The production of short-chain fatty acids, reductive enzymes, and hydrolytic enzymes by four gatifloxacin-selected, fluoroquinolone-resistant, mutant strains of C. perfringens, with stable mutations either in DNA gyrase or in both DNA gyrase and topoisomerase IV, was compared with that produced by the wild-type parent strains to investigate the effect of mutations associated with the selection of gatifloxacin resistance on bacterial metabolic activities. The mutants differed from their respective wild-type parent strains in the enzymatic activities of azoreductase, nitroreductase, and beta-glucosidase and in the ratio of butyric acid to acetic acid production. Microarray analysis of one wild type and the corresponding mutant revealed different levels of mRNA expression for the enzymes involved in short-chain fatty acid (SCFA) synthesis and for beta-glucosidase and oxidoreductases. In addition to mutations in the target genes, selection of resistance to gatifloxacin resulted in strain-specific physiological changes in the resistant mutants of C. perfringens that affected their metabolic activities.
Authors:
Fatemeh Rafii; Miseon Park; Gonçalo Gamboa da Costa; Luisa Camacho
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2009-10-24
Journal Detail:
Title:  Archives of microbiology     Volume:  191     ISSN:  1432-072X     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-03-29     Revised Date:  2013-04-24    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  895-902     Citation Subset:  IM    
Affiliation:
Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA. fatemeh.rafii@fda.hhs.gov
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / pharmacology*
Cellulases / metabolism
Clostridium perfringens / classification,  enzymology,  genetics*,  metabolism*
Drug Resistance, Bacterial / genetics*,  physiology
Fluoroquinolones / pharmacology*
Mutation
NADH, NADPH Oxidoreductases / metabolism
Nitroreductases / metabolism
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Fluoroquinolones; EC 1.6.-/NADH, NADPH Oxidoreductases; EC 1.6.-/azoreductase; EC 1.7.-/Nitroreductases; EC 3.2.1.-/Cellulases; L4618BD7KJ/gatifloxacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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