Document Detail


Comparison of manual and homogenizer methods for preparation of tick-derived stabilates of Theileria parva: equivalence testing using an in vitro titration model.
MedLine Citation:
PMID:  16038395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Theileria parva sporozoite stabilates are used in the infection and treatment method of immunization, a widely accepted control option for East Coast fever in cattle. T. parva sporozoites are extracted from infected adult Rhipicephalus appendiculatus ticks either manually, using a pestle and a mortar, or by use of an electric homogenizer. A comparison of the two methods as a function of stabilate infectivity has never been documented. This study was designed to provide a quantitative comparison of stabilates produced by the two methods. The approach was to prepare batches of stabilate by both methods and then subject them to in vitro titration. Equivalence testing was then performed on the average effective doses (ED). The ratio of infective sporozoites yielded by the two methods was found to be 1.14 in favour of the manually ground stabilate with an upper limit of the 95% confidence interval equal to 1.3. We conclude that the choice of method rests more on costs, available infrastructure and standardization than on which method produces a richer sporozoite stabilate.
Authors:
V Mbao; N Speybroeck; D Berkvens; T Dolan; P Dorny; M Madder; M Mulumba; L Duchateau; J Brandt; T Marcotty
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Parasitology     Volume:  131     ISSN:  0031-1820     ISO Abbreviation:  Parasitology     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-25     Completed Date:  2005-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401121     Medline TA:  Parasitology     Country:  England    
Other Details:
Languages:  eng     Pagination:  45-9     Citation Subset:  IM    
Affiliation:
Department of Animal Health, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Animals
Parasitology / methods
Regression Analysis
Sporozoites / isolation & purification*
Theileria parva / isolation & purification*
Ticks / parasitology*
Titrimetry

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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