Document Detail

Comparison of mammalian and fish cell line cytotoxicity: impact of endpoint and exposure duration.
MedLine Citation:
PMID:  15670629     Owner:  NLM     Status:  MEDLINE    
Comparisons of acute toxic concentrations of chemicals to fish in vivo and cytotoxic concentrations to fish cell lines in vitro reveal rather good correlations of the toxic potencies in vitro and in vivo, but a clearly lower sensitivity of the fish cells. To examine whether the low sensitivity is specific for fish cells, cytotoxic potencies of reference chemicals from the Multicenter Evaluation of In Vitro Cytotoxicity program (MEIC) reported for the fish cell lines R1 and RTG-2 were compared with those obtained with the mouse Balb/c 3T3 cell line. Cytotoxic potencies (EC(50) values) for MEIC reference chemicals were determined with exponentially growing Balb/c 3T3 cells using three different test protocols. To assess both endpoints, cell proliferation and cell survival, EC(50) values were measured for the decrease in final cell protein after 24 and 72 h of exposure and for the reduction of cell protein increase during 24 h of exposure. EC(50) values obtained with the fish cell lines R1 and RTG-2 using cell survival as endpoint were taken from the MEIC data base. The comparison of cytotoxic potencies shows that, in general, the fish cell lines and the mammalian cell line are almost equally sensitive towards the cytotoxic action of chemicals. The mammalian cell line assay, however, becomes considerably more sensitive, by factors of 3.4-8.5, than the fish cell line assays, if cell growth instead of cell survival is used as endpoint. It is concluded, that cell proliferation might be a better endpoint than cell survival and that mammalian cell lines might be suited to assess fish acute toxicity.
Michael Gülden; Sabine Mörchel; Hasso Seibert
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2004-12-25
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  71     ISSN:  0166-445X     ISO Abbreviation:  Aquat. Toxicol.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-26     Completed Date:  2005-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  229-36     Citation Subset:  IM    
Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School Schleswig-Holstein, Campus Kiel, Brunswiker Str. 10, D-24105 Kiel, Germany.
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MeSH Terms
Acute Toxicity Tests / methods*
BALB 3T3 Cells
Cell Line
Cell Proliferation / drug effects
Cell Survival / drug effects
Endpoint Determination
Hazardous Substances / toxicity*
Inhibitory Concentration 50
Lethal Dose 50
Proteins / analysis
Time Factors
Water Pollutants, Chemical / toxicity*
Reg. No./Substance:
0/Hazardous Substances; 0/Proteins; 0/Water Pollutants, Chemical

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