Document Detail

Comparison of macrophage phenotype between decidua basalis and decidua parietalis by flow cytometry.
MedLine Citation:
PMID:  18353434     Owner:  NLM     Status:  MEDLINE    
The two regions of the maternal decidua, decidua basalis and decidua parietalis, differ in the extent of trophoblast invasion and consequently in cytokines and other biological mediators, extracellular matrix and cellular components. Our aim was to compare the phenotypic features of macrophages from the two decidual regions across a broad gestational age range. We isolated macrophages by enzymatic digestion from healthy decidua samples obtained after elective abortions, at 9-18-week and at 19-23-weeks, or after term deliveries (caesarean sections at term and spontaneous term vaginal deliveries). Macrophages were analysed by flow cytometry applying the same instrument settings to all the samples to allow semi-quantitative comparison of the expression of a particular marker between different samples. We found higher expressions of CD80, CD86 and HLA-DR, suggestive of a more activated phenotype of decidual macrophages, at early/mid pregnancy than at term. Marginal differences were found between term decidual macrophages obtained after spontaneous vaginal deliveries or caesarean sections which imply that the parturient process is not associated with decidual macrophage activation. The expressions of CD105, DC-SIGN and MMR were the strongest in decidua basalis of mid pregnancy and indicate the importance of decidual macrophages in tissue homeostasis at the uteroplacental interface.
U Repnik; T Tilburgs; D L Roelen; B J van der Mast; H H H Kanhai; S Scherjon; F H J Claas
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-18
Journal Detail:
Title:  Placenta     Volume:  29     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-25     Completed Date:  2008-08-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  405-12     Citation Subset:  IM    
Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre, Albinusdreef 2 E3-Q, 2333 ZA Leiden, The Netherlands.
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MeSH Terms
Antigens, CD / metabolism
Antigens, CD14 / metabolism
Antigens, CD80 / metabolism
Antigens, CD86 / metabolism
Biological Markers / metabolism
Cell Adhesion Molecules / metabolism
Decidua / cytology*,  physiology*
Flow Cytometry
Gestational Age
HLA-DR Antigens / metabolism
Homeostasis / physiology
Lectins, C-Type / metabolism
Macrophages / metabolism,  physiology*
Mannose-Binding Lectins / metabolism
Receptors, Cell Surface / metabolism
Time Factors
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD14; 0/Antigens, CD80; 0/Antigens, CD86; 0/Biological Markers; 0/CD86 protein, human; 0/Cell Adhesion Molecules; 0/DC-specific ICAM-3 grabbing nonintegrin; 0/ENG protein, human; 0/HLA-DR Antigens; 0/Lectins, C-Type; 0/Mannose-Binding Lectins; 0/Receptors, Cell Surface; 0/mannose receptor

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