Document Detail


Comparison of mRNA levels of three ethylene receptors in senescing flowers of carnation (Dianthus caryophyllus L.).
MedLine Citation:
PMID:  11847237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Three ethylene receptor genes, DC-ERS1, DC-ERS2 and DC-ETR1, were previously identified in carnation (Dianthus caryophyllus L.). Here, the presence of mRNAs for respective genes in flower tissues and their changes during flower senescence are investigated by Northern blot analysis. DC-ERS2 and DC-ETR1 mRNAs were present in considerable amounts in petals, ovaries and styles of the flower at the full-opening stage. In the petals the level of DC-ERS2 mRNA showed a decreasing trend toward the late stage of flower senescence, whereas it increased slightly in ovaries and was unchanged in styles throughout the senescence period. However, DC-ETR1 mRNA showed no or little changes in any of the tissues during senescence. Exogenously applied ethylene did not affect the levels of DC-ERS2 and DC-ETR1 mRNAs in petals. Ethylene production in the flowers was blocked by treatment with 1,1-dimethyl-4-(phenylsulphonyl)semicarbazide (DPSS), but the mRNA levels for DC-ERS2 and DC-ETR1 decreased in the petals. DC-ERS1 mRNA was not detected in any cases. These results indicate that DC-ERS2 and DC-ETR1 are ethylene receptor genes responsible for ethylene perception and that their expression is regulated in a tissue-specific manner and independently of ethylene in carnation flowers during senescence.
Authors:
Kenichi Shibuya; Masayasu Nagata; Natsu Tanikawa; Toshihito Yoshioka; Teruyoshi Hashiba; Shigeru Satoh
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of experimental botany     Volume:  53     ISSN:  0022-0957     ISO Abbreviation:  J. Exp. Bot.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-02-15     Completed Date:  2002-05-29     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9882906     Medline TA:  J Exp Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  399-406     Citation Subset:  IM    
Affiliation:
Laboratory of Bio-adaptation, Graduate School of Agricultural Sciences, Tohoku University, Tsutsumidori-amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / physiology
Blotting, Northern
Blotting, Southern
Cloning, Molecular
Dianthus / genetics*,  growth & development,  metabolism
Ethylenes / antagonists & inhibitors,  metabolism,  pharmacology
Gene Expression Regulation, Developmental / drug effects
Gene Expression Regulation, Plant / drug effects
Plant Proteins / genetics*,  metabolism
Plant Structures / genetics*,  growth & development,  metabolism
RNA, Messenger / drug effects,  genetics*,  metabolism
Receptors, Cell Surface / genetics*,  metabolism
Semicarbazides / pharmacology
Chemical
Reg. No./Substance:
0/ERS2 protein, Dianthus; 0/Ethylenes; 0/Plant Proteins; 0/RNA, Messenger; 0/Receptors, Cell Surface; 0/Semicarbazides; 0/ethylene receptors, plant; 74-85-1/ethylene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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