Document Detail

Comparison of light-transmission and -backscattering methods in the measurement of red blood cell aggregation.
MedLine Citation:
PMID:  20459277     Owner:  NLM     Status:  MEDLINE    
Light-transmission and light-backscattering methods are commonly used to determine red-blood-cell (RBC) aggregation. Even though the results reveal good correlations between the parameters that are measured by these two methods, the methods themselves yield quite different values. The objective of this research is to investigate and delineate the characteristics of the two optical methods. We measured RBC aggregation by using a newly developed microchip-based aggregometer. An orthogonal polarization technique, wherein multiple scattering causes polarized light to be depolarized and passed through an orthogonal polarizer, was applied to the backscattering method. Our results were also compared to those of conventional aggregometers [laser-assisted optical rotational cell analyzer (LORCA)], and revealed that the backscattering method yielded higher aggregation indices than the transmission method and LORCA. However, the backscattering method with orthogonal polarization yielded the same values of aggregation indices as the transmission method. These agreements between the two methods were also found in measurements of RBC aggregability in various concentrations of dextran solutions.
Jeong-Hun Nam; Yijie Yang; Seok Chung; Sehyun Shin
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  15     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-05-12     Completed Date:  2010-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  027003     Citation Subset:  IM    
Korea University, School of Mechanical Engineering, Anam-dong, Seongbuk-gu, Seoul, Korea 136-713.
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MeSH Terms
Cells, Cultured
Erythrocyte Aggregation / physiology*
Nephelometry and Turbidimetry / methods*
Reproducibility of Results
Scattering, Radiation
Sensitivity and Specificity

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