| Comparison of lavaged and intrapulmonary alveolar macrophages in respect to lysozyme content and size in the rat. | |
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MedLine Citation:
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PMID: 6703473 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lavaged and in situ rat alveolar macrophages were compared with respect to lysozyme content and size in order to assess the extent to which macrophages from pulmonary lavages reflect the in situ cell population. This relationship was studied in normal rats and in rats with pulmonary granulomas induced by glucan stimulation (10 mg/kg given intravenously on Days 5, 3, and 1 before being killed). Alveolar macrophages in pulmonary lavages and histologic sections were stained for lysozyme by the immunoperoxidase method using rabbit antiserum to rat lysozyme. Enzyme content and cell size were measured with a conventional scanning cytospectrophotometer and an automated image analysis system (LEYTAS). Scanning cytospectrophotometry measurements showed that 26% of in situ alveolar macrophages from glucan-treated rats contained more lysozyme than did control cells and that 31% possessed larger areas. Fewer large alveolar macrophages containing increased amounts of lysozyme were detected in lavages of glucan-treated rats. Frequency histograms of lysozyme content and cell size were similar for lavaged and in situ macrophages from control rats. Measurements with LEYTAS confirmed the results. These experiments demonstrate that alveolar macrophages obtained by lavage are representative of their in situ counterparts in normal but not in glucan-treated rats. |
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Authors:
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A Kimura; E Goldstein; R M Donovan; N P Verwoerd; J S Ploem |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American review of respiratory disease Volume: 129 ISSN: 0003-0805 ISO Abbreviation: Am. Rev. Respir. Dis. Publication Date: 1984 Jan |
Date Detail:
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Created Date: 1984-04-02 Completed Date: 1984-04-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370523 Medline TA: Am Rev Respir Dis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 149-54 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Granuloma / enzymology, pathology Irrigation Lung Diseases / enzymology, pathology Macrophages / cytology, enzymology*, pathology Male Muramidase / metabolism* Pulmonary Alveoli / cytology* Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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ES-00628/ES/NIEHS NIH HHS; ES-01327/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.2.1.17/Muramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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