Document Detail


Comparison of lateral thermal injury and healing of porcine skin incisions performed by CO2-laser, monopolar electrosurgery and radiosurgery: a preliminary study based on histological and immunohistochemical results.
MedLine Citation:
PMID:  22788819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Despite the wide application of lasers and electrosurgery in dermatology, the pertinent literature provides conflicting data regarding the lateral thermal injury (LTI) associated with these instruments and its effects on wound healing. This study aims to quantitate the LTI produced by CO(2)-laser, monopolar electrosurgery (MES), and radiosurgery (MRS) and determine its effects on the healing process (re-epithelialization and inflammatory response) of incisional wounds.
METHODS: Five adult swine of similar weight (22.8-25 kg) were submitted to standardized full-thickness incisions on the lateral abdominal skin by the above instruments (at settings similar to those used in clinical practice) and scalpel (control group). Full-thickness specimens from the surgical site were harvested immediately afterwards and 48 hours later (days 1 and 3). The animals were euthanized by intravenous administration of propofol and pentobarbital. All specimens were formalin fixed, paraffin embedded, cut, and stained with hematoxylin-eosin to quantitate the extent of LTI and inflammatory infiltration. Sections of day 3 were stained with the MIB-1 monoclonal antibody to detect Ki-67 as a marker of epithelial cell proliferation adjacently to the incisions.
RESULTS: LTI was most extensive in the CO(2)-laser-group but did not differ significantly between MES- and MRS-groups. Immunohistochemistry ascertained significantly greater epithelial cell proliferation in the CO(2)-laser-group. Inflammatory infiltration was significantly greater in the CO(2)-laser-group, when compared with the controls but did not differ significantly between the MES/MRS and control groups.
CONCLUSION: CO(2) laser incisions exhibit more extensive LTI, epithelial cell proliferation, and inflammatory response. Confirmation of these findings requires a greater sample.
Authors:
Ourania K Schoinohoriti; Evanthia Chrysomali; Fotis Tzerbos; Ioannis Iatrou
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  International journal of dermatology     Volume:  51     ISSN:  1365-4632     ISO Abbreviation:  Int. J. Dermatol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-13     Completed Date:  2012-12-10     Revised Date:  2013-01-10    
Medline Journal Info:
Nlm Unique ID:  0243704     Medline TA:  Int J Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  979-86     Citation Subset:  IM    
Copyright Information:
© 2012 The International Society of Dermatology.
Affiliation:
Department of Oral Pathology and Medicine, Dental School, University of Athens, Athens, Greece. our_schoinohoriti@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Abdomen / pathology,  surgery
Animals
Burns / etiology*,  pathology,  physiopathology
Cell Proliferation
Dermatologic Surgical Procedures / adverse effects*,  instrumentation
Electrosurgery / adverse effects*,  instrumentation,  methods
Female
Humans
Inflammation / pathology,  surgery
Ki-67 Antigen / analysis
Laser Therapy / adverse effects*,  instrumentation,  methods
Lasers, Gas / adverse effects*
Male
Radiosurgery / adverse effects,  instrumentation,  methods*
Swine
Wound Healing*
Chemical
Reg. No./Substance:
0/Ki-67 Antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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