Document Detail


Comparison of laboratory-based and phylogenetic methods to distinguish between Haemophilus influenzae and H. haemolyticus.
MedLine Citation:
PMID:  18652852     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
New methods to distinguish between nontypeable Haemophilus influenzae and nonhemolytic H. haemolyticus were compared. The results of iga variable region hybridization to dotblots and library-on-a-slide microarrays were more similar to a "gold standard" multigenephylogenetic tree than iga-conserved region hybridization or P6 7F3 epitope immunoblots.
Authors:
Sara A Sandstedt; Lixin Zhang; Mayurika Patel; Kirk W McCrea; Zhaohui Qin; Carl F Marrs; Janet R Gilsdorf
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2008-07-03
Journal Detail:
Title:  Journal of microbiological methods     Volume:  75     ISSN:  0167-7012     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-08     Completed Date:  2008-11-24     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  369-71     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Outer Membrane Proteins / chemistry
Carrier State / microbiology*
Child, Preschool
Epitopes / analysis
Haemophilus / classification*,  genetics,  isolation & purification
Haemophilus Vaccines / chemistry
Haemophilus influenzae / classification*,  genetics,  isolation & purification
Humans
Immunoblotting / methods*
Nucleic Acid Hybridization / methods
Oligonucleotide Array Sequence Analysis / methods*
Pharynx / microbiology
Phylogeny*
Serine Endopeptidases / genetics*
Grant Support
ID/Acronym/Agency:
DC 05840/DC/NIDCD NIH HHS; HL 083893/HL/NHLBI NIH HHS; R01 DC005840/DC/NIDCD NIH HHS; R01 DC005840-06A1/DC/NIDCD NIH HHS; R01 HL083893/HL/NHLBI NIH HHS; R01 HL083893-03/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Epitopes; 0/Haemophilus Vaccines; 0/P6 outer membrane protein, Haemophilus; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.72/IgA-specific serine endopeptidase
Comments/Corrections

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