Document Detail

Comparison of inflammatory responses in human cells caused by lipopolysaccharides from Escherichia coli and from indigenous bacteria in aquatic environment.
MedLine Citation:
PMID:  22870993     Owner:  NLM     Status:  In-Data-Review    
The endotoxic activities of lipopolysaccharides (LPSs) in water samples are usually determined using a Limulus amoebocyte lysate (LAL) assay, but it is known that the determined activities do not always represent their inflammatory potency in humans. In this investigation, the inflammatory responses in three different human cells stimulated with Escherichia coli LPS, keratinocyte, CD14(+) monocyte, and THP-1, were compared using cytokine secretion as biomarkers to develop novel in vitro assay systems for detecting changes in inflammatory potencies of endotoxins in aquatic environment. Only THP-1 with 6-h stimulation showed dose-dependent responses in the range of normal endotoxin levels in aquatic environment. Then, the inflammatory potency of environmental LPS, which was purified from river water, was tested using THP-1. The levels and patterns of cytokine secretion after the environmental LPS stimulation were completely different from E. coli LPS. Interleukin 8 (IL-8) secretions after the environmental LPS stimulation were approximately 10-fold higher than those after E. coli LPS stimulation. The environmental LPS also induced much higher levels of TNF-α secretions in THP-1. These results suggest that a diversity of LPS structures in aquatic environment could contribute to stronger and different inflammatory responses. This investigation indicated that the proposed THP-1 assay system could be useful for detecting the changes in inflammatory potencies caused by aquatic bacteria.
Yumiko Ohkouchi; Satoshi Tajima; Masahiro Nomura; Sadahiko Itoh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering     Volume:  47     ISSN:  1532-4117     ISO Abbreviation:  J Environ Sci Health A Tox Hazard Subst Environ Eng     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-08-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9812551     Medline TA:  J Environ Sci Health A Tox Hazard Subst Environ Eng     Country:  England    
Other Details:
Languages:  eng     Pagination:  1966-74     Citation Subset:  IM    
a Department of Global Ecology, Graduate School of Global Environmental Studies , Kyoto University , Nishikyo-ku , Kyoto , Japan.
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