Document Detail


Comparison of the indirect immunofluorescence assay with western blot for the detection of HIV antibody.
MedLine Citation:
PMID:  7927564     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
With the increase in the prevalence of human immunodeficiency virus (HIV) infection in India, there is an urgent need to explore cost-containing methods of HIV antibody detection. We compared the indirect immunofluorescence assay (IFA) with the widely used but expensive confirmatory test, the Western blot (WBT). A panel of sera comprising 42 WB positive, 46 antibody negative (27 ELISA negative and 19 ELISA reactive but WB negative) and 58 sera with indeterminate WB profiles were tested by a commercial IFA kit. The sensitivity of IFA was found to be 97.6 per cent and specificity 97.8 per cent. Of the indeterminate sera, 18 (31%) were positive in IFA while 26 (45%) were negative and 14 (24%) were equivocal. In general, the IFA positivity was associated with the presence of the gag and env antibodies of HIV while negativity associated with the presence of lone antibodies. The cost of testing by IFA is less than 20 per cent of that of WB testing. Thus IFA appears to be a cost-containing alternative to WB in centres in India where immunofluorescent microscopy is routinely done.
Authors:
P Abraham; P G Babu; T J John
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Indian journal of medical research     Volume:  99     ISSN:  0971-5916     ISO Abbreviation:  Indian J. Med. Res.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-10-31     Completed Date:  1994-10-31     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0374701     Medline TA:  Indian J Med Res     Country:  INDIA    
Other Details:
Languages:  eng     Pagination:  143-8     Citation Subset:  IM; X    
Affiliation:
Department of Microbiology, Christian Medical College Hospital, Vellore.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western*
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique*
HIV Antibodies / analysis*
HIV Infections / diagnosis,  immunology
HIV-1 / immunology
Humans
Male
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/HIV Antibodies

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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