| Comparison of in vivo postexercise phosphocreatine recovery and resting ATP synthesis flux for the assessment of skeletal muscle mitochondrial function. | |
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MedLine Citation:
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PMID: 20668212 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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(31)P magnetic resonance spectroscopy (MRS) has been used to assess skeletal muscle mitochondrial function in vivo by measuring 1) phosphocreatine (PCr) recovery after exercise or 2) resting ATP synthesis flux with saturation transfer (ST). In this study, we compared both parameters in a rat model of mitochondrial dysfunction with the aim of establishing the most appropriate method for the assessment of in vivo muscle mitochondrial function. Mitochondrial dysfunction was induced in adult Wistar rats by daily subcutaneous injections with the complex I inhibitor diphenyleneiodonium (DPI) for 2 wk. In vivo (31)P MRS measurements were supplemented by in vitro measurements of oxygen consumption in isolated mitochondria. Two weeks of DPI treatment induced mitochondrial dysfunction, as evidenced by a 20% lower maximal ADP-stimulated oxygen consumption rate in isolated mitochondria from DPI-treated rats oxidizing pyruvate plus malate. This was paralleled by a 46% decrease in in vivo oxidative capacity, determined from postexercise PCr recovery. Interestingly, no significant difference in resting, ST-based ATP synthesis flux was observed between DPI-treated rats and controls. These results show that PCr recovery after exercise has a more direct relationship with skeletal muscle mitochondrial function than the ATP synthesis flux measured with (31)P ST MRS in the resting state. |
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Authors:
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N M A van den Broek; J Ciapaite; K Nicolay; J J Prompers |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-28 |
Journal Detail:
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Title: American journal of physiology. Cell physiology Volume: 299 ISSN: 1522-1563 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-28 Completed Date: 2010-11-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: C1136-43 Citation Subset: IM |
Affiliation:
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Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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metabolism Adenosine Triphosphate / biosynthesis* Animals Enzyme Inhibitors / pharmacology Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Male Mitochondria, Muscle / drug effects, metabolism* Muscle, Skeletal / metabolism*, ultrastructure Onium Compounds / pharmacology Oxidative Phosphorylation Oxygen Consumption Phosphocreatine / metabolism* Physical Conditioning, Animal / physiology* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Onium Compounds; 244-54-2/diphenyleneiodonium; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 67-07-2/Phosphocreatine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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