| Comparison of in vitro drug metabolism by lung, liver, and kidney of several common laboratory species. | |
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MedLine Citation:
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PMID: 240655 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Comparative studies of in vitro drug metabolism by hepatic and extrahepatic tissues have been complicated by the use of a single experimental tissue, few animal species, and variable experimental conditions. In an attempt to minimize these complications, liver, lung and kidney from rat, mouse, rabbit, hamster, and guinea pig were assayed for standard microsomal and soluble fraction enzymes involved in drug biotransformation. For all species, liver was the most active organ. Kidney and lung activities were usually 15%-40% of those found in liver, with kidney slightly more active than lung. No single species demonstrated total superiority in its drug-metabolizing ability, although hamster showed a large number of instances of greatest activity. The rat was a surprisingly poor representative of drug-metabolizing ability; it was superior to the other four species in less than 25% of the instances studied. All species appeared to N-demethylate aminopyrine equally except for high pulmonary and nearly absent renal activities in rabbit and high hepatic activity in hamster. Rat had the lowest level of cytochrome P-450 and low activity of NADPH-cytochrome c reductase. UDP-glucuronyltransferase activity toward the acceptors p-nitrophenol and o-aminophenol was higher in hamster and rabbit than other species. Guinea pig appeared to have the most active soluble fraction enzymes. Mouse lung and kidney had glutathione S-aryltransferase activities 10-fold greater than any other species and comparable to liver activity from rabbit and hamster. |
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Authors:
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C L Litterst; E G Mimnaugh; R L Reagan; T E Gram |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article |
Journal Detail:
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Title: Drug metabolism and disposition: the biological fate of chemicals Volume: 3 ISSN: 0090-9556 ISO Abbreviation: Drug Metab. Dispos. Publication Date: 1975 Jul-Aug |
Date Detail:
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Created Date: 1975-12-28 Completed Date: 1975-12-28 Revised Date: 2009-10-27 |
Medline Journal Info:
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Nlm Unique ID: 9421550 Medline TA: Drug Metab Dispos Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 259-65 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetyltransferases
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metabolism Aminopyrine N-Demethylase / metabolism Aniline Hydroxylase / metabolism Animals Aryl Hydrocarbon Hydroxylases / metabolism Cricetinae Cytochrome P-450 Enzyme System / metabolism Glucuronosyltransferase / metabolism Guinea Pigs Kidney / metabolism*, ultrastructure Liver / metabolism*, ultrastructure Lung / metabolism*, ultrastructure Male Mice Mice, Inbred Strains Microscopy, Electron Microsomes / enzymology, metabolism Microsomes, Liver / enzymology, metabolism NADPH-Ferrihemoprotein Reductase / metabolism Pharmaceutical Preparations / metabolism* Rabbits Rats Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Pharmaceutical Preparations; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.14.-/Aniline Hydroxylase; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.5.3.-/Aminopyrine N-Demethylase; EC 1.6.2.4/NADPH-Ferrihemoprotein Reductase; EC 2.3.1.-/Acetyltransferases; EC 2.4.1.17/Glucuronosyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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