Document Detail

Comparison of in vitro development of porcine nuclear-transferred oocytes receiving fetal somatic cells by injection and fusion methods.
MedLine Citation:
PMID:  15268779     Owner:  NLM     Status:  MEDLINE    
The present study compared the potential of nuclear-transferred porcine oocytes receiving fetal somatic cells by direct injection and cell fusion procedures to develop into blastocysts. After brief treatment of in vitro matured oocytes with demecolcine and sucrose, the protrusion containing the condensed chromosome mass was mechanically removed. Single donor cells were fused with enucleated oocytes following electric pulses or injected into oocytes by piezo-actuated microinjection. The reconstruction rate by direct injection was significantly higher than that following cell fusion (89 vs. 48%). The potential of nuclear-transferred oocytes to develop into blastocysts, however, was not different between injection and fusion methods (13% vs. 18%). Total cell number, inner cell mass, and trophectoderm cell numbers of cloned blastocysts were also not different between the two groups.
Kojiro Kawano; Yoko Kato; Yukio Tsunoda
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cloning and stem cells     Volume:  6     ISSN:  1536-2302     ISO Abbreviation:  Cloning Stem Cells     Publication Date:  2004  
Date Detail:
Created Date:  2004-07-22     Completed Date:  2005-11-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101125444     Medline TA:  Cloning Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  67-72     Citation Subset:  IM    
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, Japan.
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MeSH Terms
Blastocyst / cytology*
Cell Fusion
Cloning, Organism
Nuclear Transfer Techniques
Oocytes / cytology*

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