| Comparison of immunogenicities of recombinant Plasmodium vivax merozoite surface protein 1 19- and 42-kiloDalton fragments expressed in Escherichia coli. | |
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MedLine Citation:
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PMID: 15385477 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The 42- and 19-kDa C-terminal fragments of merozoite surface protein 1 (MSP-1(42) and MSP-1(19), respectively) are both promising blood-stage vaccine candidate antigens. At present, it is not clear which of the two antigens will be more suitable for inclusion in a cocktail malaria vaccine. In the present study, we expressed the two C-terminal fragments of Plasmodium vivax MSP-1 (PvMSP-1) in an Escherichia coli expression system and purified them by using a rapid two-step protocol. Both of the products were recognized by monoclonal antibodies against PvMSP-1 as well as by immune sera from several individuals exposed to P. vivax. We analyzed and compared the immunological responses to recombinant PvMSP-1(19) and PvMSP-1(42) in mice by using six different adjuvant formulations. Moderate to high antibody responses were observed with both of the antigens in different adjuvant formulations. Surprisingly, alum, which is generally considered to be a poor adjuvant for recombinant malaria antigens, was found to be as good an adjuvant as Montanide ISA 720, ASO2A, and other adjuvant formulations. Most adjuvant formulations induced high levels of immunoglobulin G1 (IgG1), followed by IgG3 and IgG2. Lymphocytes from animals in the PvMSP-1(42)- and PvMSP-1(19)-immunized groups showed proliferative responses upon stimulation with the respective antigens, and high levels of interleukin-4 (IL-4), IL-5, and gamma interferon were detected in the culture supernatants. Immunodepletion studies with sera from mice immunized with these two antigens showed that while immunization with PvMSP-1(42) does produce a PvMSP-1(19)-specific response, a substantial portion is also focused on structures in PvMSP-1(42) not represented by the epidermal growth factor-like domains of PvMSP-1(19). These findings may have implications for the design of MSP-1-based vaccine constructs. |
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Authors:
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Suraksha Sachdeva; Gul Ahmad; Pawan Malhotra; Paushali Mukherjee; V S Chauhan |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Infection and immunity Volume: 72 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-23 Completed Date: 2004-10-25 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 5775-82 Citation Subset: IM |
Affiliation:
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International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India. virander@icgeb.res.in |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adjuvants, Immunologic Animals Antibodies, Protozoan / analysis, immunology Enzyme-Linked Immunosorbent Assay Epitopes / immunology Escherichia coli / genetics* Humans Immunoglobulin G / analysis, immunology Immunoglobulin Isotypes / analysis, immunology Malaria Vaccines / immunology Malaria, Vivax / immunology Merozoite Surface Protein 1 / chemistry*, genetics, immunology*, isolation & purification Mice Mice, Inbred BALB C Molecular Weight Peptide Fragments / chemistry*, genetics, immunology*, isolation & purification Plasmodium vivax / immunology* Recombinant Proteins / chemistry, genetics, immunology, isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Adjuvants, Immunologic; 0/Antibodies, Protozoan; 0/Epitopes; 0/Immunoglobulin G; 0/Immunoglobulin Isotypes; 0/Malaria Vaccines; 0/Merozoite Surface Protein 1; 0/Peptide Fragments; 0/Recombinant Proteins |
| Comments/Corrections | |
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