Document Detail


Comparison of hydroxyurea and excess thymidine as synchronizing agents in cultures of L5178Y mouse lymphoma cells.
MedLine Citation:
PMID:  908455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Comparison of double hydroxyurea and hydroxyurea-excess thymidine blockades as synchronizing procedures in cultured L5178Y cells revealed that hydroxyurea mimics the effect of thymidine as regards the degree of the resulting synchrony evaluated by the mitotic index and 3H-thymidine incorporating activity. However, the peaks of the mitotic and 3H-incorporating activity appear later after thymidine than after hydroxyurea blockade and the initial maximum incorporation of 3H-deoxycytidine into DNA was about twice higher in thymidine- than in hydroxyurea-treated cells. On the other hand, the timing of 14C-formate incorporation into purine DNA bases was closely similar in both cell populations.
Authors:
L Kornacka; J Sawecka; J Malec
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Folia biologica     Volume:  23     ISSN:  0015-5500     ISO Abbreviation:  Folia Biol. (Praha)     Publication Date:  1977  
Date Detail:
Created Date:  1977-11-30     Completed Date:  1977-11-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0234640     Medline TA:  Folia Biol (Praha)     Country:  CZECHOSLOVAKIA    
Other Details:
Languages:  eng     Pagination:  299-304     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Radioisotopes
Cell Cycle / drug effects
Cell Membrane Permeability / drug effects
Cells, Cultured
DNA, Neoplasm / biosynthesis
Hydroxyurea / pharmacology*
Lymphoma / metabolism,  physiopathology*
Mice
Mice, Inbred Strains
Mitosis / drug effects
Thymidine / pharmacology*
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/DNA, Neoplasm; 127-07-1/Hydroxyurea; 50-89-5/Thymidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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