Document Detail

Comparison of human chorionic gonadotrophin in human urine and amniotic fluid as estimated by a polyclonal and by a specific monoclonal assay in very early pregnancy.
MedLine Citation:
PMID:  2606964     Owner:  NLM     Status:  MEDLINE    
Urinary human chorionic gonadotrophin (HCG) was estimated in 42 samples from patients in very early pregnancy or shortly after early spontaneous abortion, in which initial testing showed a positive urinary HCG in the Hybritech Tandem ICON II monoclonal 2 min test. There were no false positives with this test even when the blue colour was difficult to distinguish from the white background, i.e. considerably less than the calibration blue spot at 50 mIU/ml. The Serono polyclonal beta-HCG radioimmunoassay (RIA) detected urinary intact HCG (plus free beta-subunit) at concentrations as low as 10 mIU/ml IS 61/6 for bioassay (the old standard), i.e. approximately 22 mIU/ml 1st IRP 75/537 for immunoassay (the new standard). The LKB-Pharmacia DELFIA monoclonal fluoroimmunoassay (FIA) detected urinary intact HCG, without free beta-subunit, to a minimum concentration of 18 mIU/ml 75/537. The proportion of monoclonal/polyclonal was 2-155%. In normal amniotic fluid at 16-20 weeks of pregnancy, HCG concentrations were 3180 +/- 2270 (755-11,000) mIU/ml with the FIA.
J Brotherton
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  4     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  1989 Oct 
Date Detail:
Created Date:  1990-02-09     Completed Date:  1990-02-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  837-42     Citation Subset:  IM    
Department of Gynaecological Endocrinology, Sterility and Family Planning, Free University, Berlin, FRG.
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MeSH Terms
Amniotic Fluid / analysis*
Antibodies, Monoclonal / diagnostic use*
Chorionic Gonadotropin / analysis*,  urine
Pregnancy Trimester, First
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Chorionic Gonadotropin

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