Document Detail


Comparison of host tissue and bacterial dipeptidyl peptidases in human gingival crevicular fluid by analytical isoelectric focusing.
MedLine Citation:
PMID:  7487574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Earlier work has shown that gingival crevicular fluid (GCF) contains dipeptidyl peptidase (DPP) activities that resemble those in host tissue. Here, further comparisons were made with enzymes from suspected periodontal pathogens. Gingival tissue and GCF were collected from patients with chronic periodontitis. DPP II and DPP IV fractions with acid and alkaline pH optima, respectively, were separated from crude tissue extracts by gel-filtration chromatography. Bacterial cell sonicates were prepared from broth cultures of reference strains. There was moderate to strong DPP activity with Capnocytophaga spp., Porphyromonas gingivalis and Prevotella spp., very weak activity with Treponema denticola and no detectable activity with Actinobacillus actinomycetemcomitans or Fusobacterium nucleatum. Banding patterns in GCF, tissue and bacterial samples were compared on substrate-impregnated overlay membranes applied to isoelectric focusing gels. In gels washed with acid buffer, GCF had bands corresponding to tissue DPP II. Use of an alkaline washing buffer showed GCF activity which closely matched tissue DPP IV that had been pretreated with neuraminidase, an enzyme found by others in the gingival crevice. P. Gingivalis gave multiple bands and several of these had counterparts in GCF. The apparent presence in GCF of the DPP from P. gingivalis is consistent with the association of this organism with destructive periodontitis.
Authors:
M I Gazi; S W Cox; D T Clark; B M Eley
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of oral biology     Volume:  40     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-11-28     Completed Date:  1995-11-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  731-6     Citation Subset:  D; IM    
Affiliation:
Department of Periodontology, King's College School of Medicine and Dentistry, Denmark Hill, London, U.K.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / analysis*
Capnocytophaga / enzymology
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / analysis*
Gingiva / enzymology
Gingival Crevicular Fluid / enzymology*
Humans
Isoelectric Focusing
Periodontitis / enzymology*,  microbiology*
Porphyromonas gingivalis / enzymology
Prevotella intermedia / enzymology
Sonication
Treponema / enzymology
Chemical
Reg. No./Substance:
0/Bacterial Proteins; EC 3.4.14.-/Dipeptidyl-Peptidases and Tripeptidyl-Peptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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