Document Detail

Comparison of hexachlorocyclohexane-induced oxidative stress in the testis of immature and adult rats.
MedLine Citation:
PMID:  9467884     Owner:  NLM     Status:  MEDLINE    
1. The acute effect of hexachlorocyclohexane (HCH) administration (i.p.) on testicular antioxidant system and lipid peroxidation (LPX) in immature and mature rats (15- and 90-day-old, respectively) were compared. 2. In both the age groups, the level of LPX in crude homogenate of testis (endogenous, as well as FeSO4, and ascorbic acid-stimulated) was increased after 6 hr of HCH treatment and remained high till 24 hr. However, FeSO4 and ascorbic acid-stimulated LPX was higher in 90-day-old rats in comparison to 15-day-old rats. HCH treatment also resulted in elevation of LPX level in testicular subcellular (nuclear, mitochondrial and microsomal) fractions by 6 hr of treatment. However, the magnitude of increase was greater in case of 90-day-old rats. 3. Activities of testicular cytosolic superoxide dismutases (total and CN(-)-resistant) of rats of 15- and 90-day-old age groups decreased significantly after 6 hr of HCH treatment, and remained decreased till 24 hr of the pesticide treatment. The percentage of decrease was higher in 15-day-old rats than 90-day-old rats. CN(-)-sensitive SOD activity of testis was found to decrease by 12 and 24 hr after the pesticide treatment in 15- and 90-day-old rats, respectively. The activity of catalase decreased 6 hr after the pesticide treatment in both the age groups. However, the magnitude of decrease was similar for both age groups of rats. 4. Testicular glutathione content, as well as levels of glutathione metabolizing enzymes (glutathione peroxidase and glutathione reductase), did not change in response to HCH treatment, whereas ascorbic acid content decreased by 12 and 6 hr after HCH treatment in 15- and 90-day-old rats, respectively. The level of H2O2 was found to be elevated after 6 hr of the pesticide treatment in both age groups. 5. Total epididymal sperm number was comparable in all experimental groups. However, the percentage of dead and damaged spermatozoa was significantly enhanced in HCH treated rats. 6. Acute HCH administration to rats results in induction of oxidative stress in the testis which depends upon the age of the animal.
L Samanta; G B Chainy
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology     Volume:  118     ISSN:  1367-8280     ISO Abbreviation:  Comp. Biochem. Physiol. C, Pharmacol. Toxicol. Endocrinol.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-05-05     Completed Date:  1998-05-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9516060     Medline TA:  Comp Biochem Physiol C Pharmacol Toxicol Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  319-27     Citation Subset:  IM    
Department of Zoology, Utkal University, Bhubaneswar, India.
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MeSH Terms
Age Factors
Ascorbic Acid / pharmacology
Carcinogens / toxicity*
Catalase / metabolism
Ferrous Compounds / pharmacology
Glutathione / metabolism
Glutathione Peroxidase / metabolism
Glutathione Reductase / metabolism
Hydrogen Peroxide / metabolism
Insecticides / toxicity*
Lindane / toxicity*
Lipid Peroxidation / drug effects
Oxidative Stress / drug effects*
Sperm Count / drug effects
Superoxide Dismutase / metabolism
Testis / drug effects*,  enzymology
Time Factors
Reg. No./Substance:
0/Carcinogens; 0/Ferrous Compounds; 0/Insecticides; 50-81-7/Ascorbic Acid; 58-89-9/Lindane; 70-18-8/Glutathione; 7720-78-7/ferrous sulfate; 7722-84-1/Hydrogen Peroxide; EC; EC Peroxidase; EC Dismutase; EC Reductase

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