| Comparison of the glutamate-, arginine- and lysine-dependent acid resistance systems in Escherichia coli O157:H7. | |
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MedLine Citation:
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PMID: 15139915 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: The objective of this study was to investigate the effect of growing conditions on the glutamate-, arginine- and lysine-dependent acid resistance (AR) systems of Escherichia coli O157:H7. METHODS AND RESULTS: Seven E. coli O157:H7 strains were grown in five different media at neutral or acidic pH under aerobic or anaerobic conditions, and the survival rate after acid shocks (pH 2.0, 1 h, 37 degrees C) in the presence of glutamate, arginine and lysine was determined. Six strains induced the glutamate-dependent AR at stationary phase, and maximal survival were observed (> or =10%) when grown in pH 5- Luria-Bertani media with glucose (LBG) and in pH 4.5-anaerobic media. The arginine- and lysine-dependent systems were also present, but were only induced if cells had grown in LBG. For strain ATCC 43895, the minimum glutamate concentration that resulted in at least 10% survival rate was 10 micromol l(-1), but it required at least 10-fold more arginine and lysine. CONCLUSIONS: The lysine-dependent AR system could be as important as the arginine-mediated one, but the contribution of both systems to E. coli O157:H7 overall AR response might be minor compared with the glutamate-dependent system. SIGNIFICANCE AND IMPACT OF THE STUDY: Under typical environmental conditions, the glutamate-dependent AR system might be solely responsible for protecting cells against acidic pH. |
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Authors:
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F Diez-Gonzalez; Y Karaibrahimoglu |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of applied microbiology Volume: 96 ISSN: 1364-5072 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2004 |
Date Detail:
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Created Date: 2004-05-13 Completed Date: 2004-09-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 1237-44 Citation Subset: IM |
Affiliation:
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Department of Food Science and Nutrition, University of Minnesota, St Paul, MN, USA. fdiez@umn.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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pharmacology* Arginine / pharmacology Culture Media Dose-Response Relationship, Drug Escherichia coli O157 / drug effects*, growth & development*, physiology Food Microbiology Glutamic Acid / pharmacology Hydrogen-Ion Concentration Lysine / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Culture Media; 56-86-0/Glutamic Acid; 56-87-1/Lysine; 74-79-3/Arginine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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