Document Detail


Comparison of the glutamate-, arginine- and lysine-dependent acid resistance systems in Escherichia coli O157:H7.
MedLine Citation:
PMID:  15139915     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: The objective of this study was to investigate the effect of growing conditions on the glutamate-, arginine- and lysine-dependent acid resistance (AR) systems of Escherichia coli O157:H7. METHODS AND RESULTS: Seven E. coli O157:H7 strains were grown in five different media at neutral or acidic pH under aerobic or anaerobic conditions, and the survival rate after acid shocks (pH 2.0, 1 h, 37 degrees C) in the presence of glutamate, arginine and lysine was determined. Six strains induced the glutamate-dependent AR at stationary phase, and maximal survival were observed (> or =10%) when grown in pH 5- Luria-Bertani media with glucose (LBG) and in pH 4.5-anaerobic media. The arginine- and lysine-dependent systems were also present, but were only induced if cells had grown in LBG. For strain ATCC 43895, the minimum glutamate concentration that resulted in at least 10% survival rate was 10 micromol l(-1), but it required at least 10-fold more arginine and lysine. CONCLUSIONS: The lysine-dependent AR system could be as important as the arginine-mediated one, but the contribution of both systems to E. coli O157:H7 overall AR response might be minor compared with the glutamate-dependent system. SIGNIFICANCE AND IMPACT OF THE STUDY: Under typical environmental conditions, the glutamate-dependent AR system might be solely responsible for protecting cells against acidic pH.
Authors:
F Diez-Gonzalez; Y Karaibrahimoglu
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  96     ISSN:  1364-5072     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2004  
Date Detail:
Created Date:  2004-05-13     Completed Date:  2004-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1237-44     Citation Subset:  IM    
Affiliation:
Department of Food Science and Nutrition, University of Minnesota, St Paul, MN, USA. fdiez@umn.edu
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / pharmacology*
Arginine / pharmacology
Culture Media
Dose-Response Relationship, Drug
Escherichia coli O157 / drug effects*,  growth & development*,  physiology
Food Microbiology
Glutamic Acid / pharmacology
Hydrogen-Ion Concentration
Lysine / pharmacology
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 56-86-0/Glutamic Acid; 56-87-1/Lysine; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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