Document Detail


Comparison of glucose, fructose, ascorbic acid and glucosephosphate isomerase enzymatic activity in uterine flushings from nonpregnant and pregnant gilts and pony mares.
MedLine Citation:
PMID:  7159659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Experiment 1, 40 gilts and 30 pony mares were used to characterize changes in glucose, fructose, ascorbic acid and glucosephosphate isomerase (GPI) enzymatic activity in uterine flushings collected either during the estrous cycle or early pregnancy. Total recoverable glucose was greater (P less than 0.01) in uterine flushings from pregnant gilts, but pregnancy status had no effect on total recoverable glucose in pony mare uterine flushings. Fructose was undetectable in uterine flushings from nonpregnant gilts and pony mares and pregnant gilts and pony mares prior to Day 14, but occurred in increasing amounts between Days 14 and 18 or 20 of pregnancy. In Experiment 2, it was demonstrated that the porcine conceptus is the primary source, if not the sole source of fructose. Total recoverable ascorbic acid in uterine flushings was not affected by pregnancy in gilts, but was greater (P less than 0.01) in pregnant versus nonpregnant pony mares. In both species, total recoverable ascorbic acid was affected (P less than 0.01) by day of the estrous cycle and pregnancy. The GPI enzyme allows for the interconversion of glucose-6-PO4 and fructose-6-PO4. GPI total and specific activities were greater (P less than 0.01) for pregnant than nonpregnant gilts and pony mares. The periods of greatest GPI activity were temporally associated with elevated estrogens of either ovarian or blastocyst origin. Results from Experiment 3 indicated a marked increase (P less than 0.01) in GPI activity in uterine flushings from gilts treated with estradiol valerate. Results of this study indicate that glucose (gilt only), fructose, ascorbic acid and GPI activity are increased in uterine flushings of gilts and pony mares during early pregnancy. The increase in these constituents may reflect increased carbohydrate metabolism in ways which are uniquely beneficial to conceptus development in ungulates.
Authors:
M T Zavy; W R Clark; D C Sharp; R M Roberts; F W Bazer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  27     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1982 Dec 
Date Detail:
Created Date:  1983-04-15     Completed Date:  1983-04-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1147-58     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascorbic Acid / metabolism
Carbohydrate Metabolism*
Estrus
Female
Fructose / metabolism
Glucose / metabolism
Glucose-6-Phosphate Isomerase / metabolism
Horses / metabolism*
Pregnancy
Pregnancy, Animal*
Uterus / metabolism*
Grant Support
ID/Acronym/Agency:
HD 08560/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
30237-26-4/Fructose; 50-81-7/Ascorbic Acid; 50-99-7/Glucose; EC 5.3.1.9/Glucose-6-Phosphate Isomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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