Document Detail


Comparison of gene expression and fatty acid profiles in concentrate and forage finished beef.
MedLine Citation:
PMID:  23048149     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Fatty acid profiles and intramuscular expression of genes involved in fatty acid metabolism were characterized in concentrate- (CO) and forage- (FO) based finishing systems. Intramuscular samples from the adductor were taken at slaughter from 99 heifers finished on a CO diet and 58 heifers finished on a FO diet. Strip loins were obtained at fabrication to evaluate fatty acid profiles of LM muscle for all 157 heifers by using gas chromatography fatty acid methyl ester analysis. Composition was analyzed for differences by using the General Linear Model (GLM) procedure in SAS. Differences in fatty acid profile included a higher atherogenic index, higher percentage total MUFA, lower omega-3 to omega-6 ratio, lower percentage total PUFA, and lower percentage omega-3 fatty acids in CO- compared to FO-finished heifers (P < 0.05). Fatty acid profiles from intramuscular samples were ranked by the atherogenic index, and 20 heifers with either a high (HAI; n = 10) or low (LAI; n = 10) atherogenic index were selected for gene expression analysis using real-time PCR (RT-PCR). Gene expression data for the 20 individuals were analyzed as a 2 x 2 factorial using the GLM procedure in SAS. There was no significant diet x atherogenic index interaction identified for any gene (P > 0.05). Up-regulation was observed for peroxisome proliferator activated receptor gamma (PPARγ), fatty acid synthase (FASN), and fatty acid binding protein 4 (FABP4) in FO-finished compared to CO-finished heifers in both atherogenic index categories (P < 0.05). Up-regulation of diglyceride acyl transferase 2 (DGAT2) was observed in FO-finished heifers with a HAI (P < 0.05). Expression of steroyl Co-A desaturase (SCD) was up-regulated in CO-finished heifers with a LAI, and down-regulated in FO finished heifers with a HAI (P < 0.05). Expression of adiponectin (ADIPOQ) was significantly down-regulated in CO-finished heifers with a HAI compared to all other categories (P < 0.05). The genes identified in this study which exhibit differential regulation in response to diet or in animals with extreme fatty acid profiles may provide genetic markers for selecting desirable fatty acid profiles in future selection programs.
Authors:
J W Buchanan; A J Garmyn; G G Hilton; D L Vanoverbeke; Q Duan; D C Beitz; R G Mateescu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-9
Journal Detail:
Title:  Journal of animal science     Volume:  -     ISSN:  1525-3163     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Animal Science, Oklahoma State University, Stillwater, OK, 74078.
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