Document Detail

Comparison of gamma-radiation-induced accumulation of ataxia telangiectasia and control cells in G2 phase.
MedLine Citation:
PMID:  2549409     Owner:  NLM     Status:  MEDLINE    
Recent reports from a number of laboratories have linked radiosensitivity in ataxia telangiectasia (A-T) to a large and prolonged block of some cells in G2 phase. Previous results from this laboratory, largely with one Epstein-Barr virus-transformed A-T lymphoblastoid cell line, presented evidence for a dramatic increase in the number of cells in G2 phase over controls during a 24-h period post irradiation. We describe here a study of the effect of gamma-radiation on G2 phase delay in several A-T cell lines. Based on previous results with several cell lines 24 h post irradiation was selected as the optimum time to discriminate between G2 phase delay in control and A-T cells. All A-T homozygotes showed a significantly greater number of cells in G2 phase, 24 h post irradiation, than observed in controls. A more prolonged delay in G2 phase after irradiation was seen in different A-T cell types that included lymphoblastoid cells, fibroblasts and SV40-transformed fibroblasts. At the radiation dose used it was not possible to distinguish A-T heterozygotes from controls.
P R Bates; M F Lavin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  218     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1989-10-10     Completed Date:  1989-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  165-70     Citation Subset:  IM    
Clinical Research Centre, Royal Brisbane Hospital, Queensland, Australia.
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MeSH Terms
Ataxia Telangiectasia
Cell Line
Cell Transformation, Viral
Fibroblasts / cytology,  radiation effects
Flow Cytometry
Gamma Rays
Herpesvirus 4, Human / genetics
Interphase / radiation effects*
Simian virus 40 / genetics

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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