Document Detail


Comparison of frozen versus desiccated reference human red blood cells for hemagglutination assays.
MedLine Citation:
PMID:  19555416     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Red blood cells (RBCs) are commonly used fresh or stored in frozen format for identification of patients' antibodies and serologic specificity of such antibodies at reference laboratories. However, maintaining a large pool of fresh RBCs is impossible in a blood-banking environment and blood in frozen format poses a logistic disadvantage in terms of accessibility, maintenance cost, safety, and sample recovery. This study explores an alternative, desiccation storage method for RBCs to provide a reagent that supports greater utilization and flexibility for reference laboratories. STUDY DESIGN AND METHODS: RBCs from five donors were used in the study. RBCs were processed and kept in either frozen or desiccated format. Study variables for either the frozen or the desiccated cells included cell recovery as quantified by cell counts, gross microscopic examination, and hemagglutination assays. RESULTS: The mean percentage of cell recovery for thawed and washed frozen RBCs was 20% versus 50% for rehydrated and washed desiccated RBCs. Microscopic examination of thawed cells from the frozen preparation showed cells with irregular shapes, a sharp contrast when compared with rehydrated cells from the desiccated preparation, where cells are mostly intact, smooth surface, and biconcave in structure. Cells in both preparations performed well in manual agglutination tests. CONCLUSION: Desiccation preservation of RBCs provides a somewhat better RBC recovery and cell structure stability, while maintaining the necessary antigen-antibody reactions for cell surface markers, which will allow desiccated RBCs to be archived in blood collecting and processing reference laboratories.
Authors:
David Ho; Jennifer Schierts; Zea Zimmerman; Isaac Gadsden; Stephen Bruttig
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Publication Detail:
Type:  Journal Article     Date:  2009-06-23
Journal Detail:
Title:  Transfusion     Volume:  49     ISSN:  1537-2995     ISO Abbreviation:  Transfusion     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-11-11     Completed Date:  2009-12-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2173-80     Citation Subset:  IM    
Affiliation:
HeMemics Biotechnologies, Inc., Rockville, Maryland, USA. dho@hememics.com
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MeSH Terms
Descriptor/Qualifier:
Blood Preservation / methods*
Cryopreservation / methods*
Desiccation / methods*
Erythrocytes*
Hemagglutination Tests*
Humans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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