Document Detail

Comparison of fresh and cryopreserved porcine ventral mesencephalon cells transplanted in A rat model of Parkinson's disease.
MedLine Citation:
PMID:  12125079     Owner:  NLM     Status:  MEDLINE    
To evaluate whether cryopreservation of porcine ventral mesencephalon cells influences graft survival and function in vivo, we have transplanted either freshly prepared or cryopreserved cells into the striatum of 6-hydroxydopamine-lesioned rats. A single cell suspension of porcine ventral mesencephalon cells from the same isolation either was stored at 4 degrees C and transplanted the next day or was cryopreserved for 4 weeks in liquid nitrogen vapor. The cryopreserved cells were then rapidly thawed, rinsed, and transplanted in the same manner as the fresh cells, with the same dose of viable cells. All animals received daily injections of cyclosporin A to prevent xenograft rejection. To monitor graft function, amphetamine-induced rotation was measured every 3 weeks between 6 and 15 weeks posttransplantation. After sacrifice at 15 weeks posttransplantation, histological methods were used to compare fresh cell and cryopreserved cell transplants with respect to graft survival, differentiation and integration, and host immune response. Cryopreserved cells were found to be either equivalent or in some cases superior to fresh cells with respect to rotational correction, graft survival, graft volume, numbers of graft-derived dopaminergic neurons, and host immune responses. In conclusion, the results indicate that it is feasible to cryopreserve porcine ventral mesencephalon cells for long-term storage of cells prior to transplantation in an animal model of Parkinson's disease.
Douglas B Jacoby; Charles Lindberg; Judson Ratliff; Kristie Wetzel; Gregory R Stewart; Jonathan Dinsmore
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  69     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-18     Completed Date:  2002-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  382-96     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Wiley-Liss, Inc.
Department of Cell Transplantation, Diacrin Inc., Charlestown, Massachusetts, USA.
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MeSH Terms
Adrenergic Agents
Behavior, Animal
Cell Culture Techniques
Corpus Striatum / immunology,  surgery*
Cryopreservation* / methods
Graft Survival
Mesencephalon / cytology,  transplantation*
Models, Animal
Parkinson Disease / immunology,  pathology*,  physiopathology,  therapy*
Parkinson Disease, Secondary / chemically induced
Rats, Sprague-Dawley
Time Factors
Reg. No./Substance:
0/Adrenergic Agents; 1199-18-4/Oxidopamine

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