Document Detail

Comparison of four methods for screening of cytomegalovirus antibodies in normal donors and immunocompromised patients.
MedLine Citation:
PMID:  2822396     Owner:  NLM     Status:  MEDLINE    
Three commercial methods (enzyme immunoassay, fluoroimmunoassay and latex agglutination) and a complement fixation test were compared for detection of cytomegalovirus antibodies using a panel of 490 serum samples from blood and organ donors, from immunocompromised patients and paired sera from five patients with recent cytomegalovirus infection. An indirect immunofluorescence test for antibodies to cytomegalovirus was used for classifying samples giving discrepant results by any of the four methods. All methods showed high sensitivity and specificity, but the enzyme immunoassay and the latex agglutination tests had the highest sensitivity. Latex agglutination is recommended for large-scale screening of cytomegalovirus antibodies in blood and organ donors. Negative results obtained by latex agglutination should be confirmed by sensitive enzyme immunoassays.
F de Ory; P León; C Domingo; A Garcia-Sáiz; L Pérez; J M Echevarría
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  European journal of clinical microbiology     Volume:  6     ISSN:  0722-2211     ISO Abbreviation:  Eur. J. Clin. Microbiol.     Publication Date:  1987 Aug 
Date Detail:
Created Date:  1987-12-15     Completed Date:  1987-12-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8219582     Medline TA:  Eur J Clin Microbiol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  402-5     Citation Subset:  IM    
Servicio de Virología, Centro Nacional de Microbiología, Virología en Immunología Sanitarias, Majadahonda, Madrid, Spain.
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MeSH Terms
Antibodies, Viral / analysis*
Blood Donors
Complement Fixation Tests
Cytomegalovirus / immunology*
Cytomegalovirus Infections / diagnosis,  immunology
Fluorescent Antibody Technique
Immune Tolerance
Immunoenzyme Techniques
Latex Fixation Tests
Predictive Value of Tests
Tissue Donors
Reg. No./Substance:
0/Antibodies, Viral

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