| Comparison of four in vitro assays for specific IgE detection. | |
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MedLine Citation:
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PMID: 7919425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three immunoenzymatic techniques for specific IgE detection (Pharmacia CAP System, Kallestad Allercoat System, Neo Abellò Hamlet-IgE) and the classical Phadebas RAST were compared using 34 sera from patients with a clinical diagnosis of allergic disease and 19 sera from healthy non-atopic controls. IgE antibodies to 9 aeroallergens and 6 food antigens were assessed and 399 tests were run with each method. All techniques showed a high specificity (92%-100%) and satisfactory efficiency (82%-98%), while the sensitivity for RAST, CAP, Allercoat and Hamlet was 89%, 91%, 83% and 53%, respectively, with the lowest values for food allergens. There was a good overall correlation of the four techniques, except when the Hamlet method was compared with the other methods for food-specific IgE detection (correlation coefficient < 0.3). These data indicate that CAP, Allercoat and RAST are satisfactory techniques for specific IgE determination, either for inhalants or for food allergens; CAP, however, offers the highest sensitivity without loss of specificity. |
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Authors:
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P Boccagni; F Favari; G Zanoni; A Pezzini; G Tridente |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: International journal of clinical & laboratory research Volume: 24 ISSN: 0940-5437 ISO Abbreviation: Int. J. Clin. Lab. Res. Publication Date: 1994 |
Date Detail:
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Created Date: 1994-10-27 Completed Date: 1994-10-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9206491 Medline TA: Int J Clin Lab Res Country: GERMANY |
Other Details:
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Languages: eng Pagination: 102-5 Citation Subset: IM |
Affiliation:
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Institute of Immunology and Infectious Diseases, University of Verona, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Allergens / immunology* Female Humans Hypersensitivity / diagnosis* Immunoenzyme Techniques* Immunoglobulin E / blood* Male Middle Aged Radioallergosorbent Test* Reproducibility of Results Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 37341-29-0/Immunoglobulin E |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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