Document Detail

Comparison of four in vitro assays for specific IgE detection.
MedLine Citation:
PMID:  7919425     Owner:  NLM     Status:  MEDLINE    
Three immunoenzymatic techniques for specific IgE detection (Pharmacia CAP System, Kallestad Allercoat System, Neo Abellò Hamlet-IgE) and the classical Phadebas RAST were compared using 34 sera from patients with a clinical diagnosis of allergic disease and 19 sera from healthy non-atopic controls. IgE antibodies to 9 aeroallergens and 6 food antigens were assessed and 399 tests were run with each method. All techniques showed a high specificity (92%-100%) and satisfactory efficiency (82%-98%), while the sensitivity for RAST, CAP, Allercoat and Hamlet was 89%, 91%, 83% and 53%, respectively, with the lowest values for food allergens. There was a good overall correlation of the four techniques, except when the Hamlet method was compared with the other methods for food-specific IgE detection (correlation coefficient < 0.3). These data indicate that CAP, Allercoat and RAST are satisfactory techniques for specific IgE determination, either for inhalants or for food allergens; CAP, however, offers the highest sensitivity without loss of specificity.
P Boccagni; F Favari; G Zanoni; A Pezzini; G Tridente
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  International journal of clinical & laboratory research     Volume:  24     ISSN:  0940-5437     ISO Abbreviation:  Int. J. Clin. Lab. Res.     Publication Date:  1994  
Date Detail:
Created Date:  1994-10-27     Completed Date:  1994-10-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9206491     Medline TA:  Int J Clin Lab Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  102-5     Citation Subset:  IM    
Institute of Immunology and Infectious Diseases, University of Verona, Italy.
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MeSH Terms
Allergens / immunology*
Hypersensitivity / diagnosis*
Immunoenzyme Techniques*
Immunoglobulin E / blood*
Middle Aged
Radioallergosorbent Test*
Reproducibility of Results
Sensitivity and Specificity
Reg. No./Substance:
0/Allergens; 37341-29-0/Immunoglobulin E

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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