Document Detail


Comparison of flow cytometry and retrospectively applied static cytometry on lymphoid tissue.
MedLine Citation:
PMID:  2194157     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Twenty cases of non-Hodgkin's malignant lymphoma were examined using both flow cytometry (FCM) and static cytometry (SCM) DNA analysis to detect aneuploidic cell populations. FCM was performed on fresh cell suspensions whilst SCM was performed retrospectively on formalin-fixed, paraffin-embedded samples of the same tissue. A total of 34 aneuploidic cell lines were detected by FCM compared to a total of 41 detected by SCM. Of the 20 cases 14 showed DNA indexes within 10% of each other for each method, indicating a reasonable degree of comparability between the two methods. Of the 6 cases which did not show comparable DNA indexes, 2 displayed similar cell lines for both methods, but the major abnormal cell populations were of different ploidy by SCM as compared to FCM. A qualitative comparison is thus possible between these two cases. The results suggest that retrospectively applied SCM ploidy analysis of lymphoid tissue is comparable to FCM on fresh tissue samples and can thus be used in retrospective studies of both prognostic and diagnostic significance.
Authors:
A Jones; J Grace; B E Hall
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Pathology     Volume:  22     ISSN:  0031-3025     ISO Abbreviation:  Pathology     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-08-08     Completed Date:  1990-08-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0175411     Medline TA:  Pathology     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  5-9     Citation Subset:  IM    
Affiliation:
Department of Anatomical Pathology, Royal Prince Alfred Hospital, Sydney.
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MeSH Terms
Descriptor/Qualifier:
Cytological Techniques*
DNA, Neoplasm / genetics
Densitometry
Flow Cytometry*
Humans
Lymphoid Tissue / cytology*
Lymphoma, Non-Hodgkin / pathology
Ploidies
Chemical
Reg. No./Substance:
0/DNA, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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