Document Detail

Comparison of flow cytometric methods for the measurement of ZAP-70 expression in a routine diagnostic laboratory.
MedLine Citation:
PMID:  16048494     Owner:  NLM     Status:  MEDLINE    
Chronic lymphocytic leukaemia (CLL) follows a variable clinical course with patient survival ranging from only a few years despite treatment, to several decades in patients who may never require clinical intervention. Determination of the mutational status of a patient's immunoglobulin heavy chain variable region (Ig V(H)) gene has been used to provide prognostic information, but this assay is not available in most laboratories. The discovery of the expression of the protein tyrosine kinase zeta-associated protein (ZAP)-70 in V(H)-unmutated CLL cases led to its proposal as a surrogate marker for V(H) status. This study investigated the measurement of ZAP-70 expression in CLL using different flow cytometric protocols. Two different antibodies and two different staining methods were compared. The Caltag ZAP-70 antibody and Fix & Perm kit were the easiest to use and were the most sensitive and specific combination, with 91% concordance between ZAP-70 expression and V(H) status. Three patients (9%) were discordant (two V(H) mutated/ZAP-70 positive, and one V(H) unmutated/ZAP-70 negative). No correlation existed between CD38 and either ZAP-70 expression or V(H) status. Measurement of ZAP-70 expression using the Caltag antibody/kit combination provides a standardized flow cytometric method that could be introduced into a routine CLL immunophenotyping panel in a clinical diagnostic laboratory.
G Gibbs; T Bromidge; D Howe; J Hopkins; S Johnson
Related Documents :
25219364 - Effects of ischemia and oxidative stress on bladder purinoceptors expression.
25209444 - Human papillomavirus 16 oncoprotein regulates the translocation of β-catenin via the ac...
25231404 - Calreticulin promotes migration and invasion of esophageal cancer cells by up-regulatin...
10781684 - Identification of acute myeloid leukemia patients with diminished expression of cd13 my...
10421804 - Localization of regulatory protein binding sites in the proximal region of human myomet...
765754 - Stimulation in trans of synthesis of e. coli gal operon enzymes by lambdoid phages duri...
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical and laboratory haematology     Volume:  27     ISSN:  0141-9854     ISO Abbreviation:  Clin Lab Haematol     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-28     Completed Date:  2005-11-08     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  7907061     Medline TA:  Clin Lab Haematol     Country:  England    
Other Details:
Languages:  eng     Pagination:  258-66     Citation Subset:  IM    
Department of Haematology, Taunton & Somerset Hospital, Taunton TA1 5DA, Somerset, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Aged, 80 and over
Clinical Laboratory Techniques*
Flow Cytometry / methods*
Gene Expression Regulation, Neoplastic
Immunoglobulin Heavy Chains / genetics
Immunoglobulin Variable Region / genetics
Leukemia, Lymphocytic, Chronic, B-Cell / diagnosis*,  genetics*
Middle Aged
Protein-Tyrosine Kinases / analysis*,  genetics*
Reagent Kits, Diagnostic
Sensitivity and Specificity
ZAP-70 Protein-Tyrosine Kinase
Reg. No./Substance:
0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Variable Region; 0/Reagent Kits, Diagnostic; EC Kinases; EC Protein-Tyrosine Kinase; EC protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Platelets in the paediatric population: the influence of age and the limitations of automation.
Next Document:  Efficacy and response to intravenous anti-D immunoglobulin in chronic idiopathic thrombocytopenic pu...