Document Detail


Comparison of flow cytometric methods for the measurement of ZAP-70 expression in a routine diagnostic laboratory.
MedLine Citation:
PMID:  16048494     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chronic lymphocytic leukaemia (CLL) follows a variable clinical course with patient survival ranging from only a few years despite treatment, to several decades in patients who may never require clinical intervention. Determination of the mutational status of a patient's immunoglobulin heavy chain variable region (Ig V(H)) gene has been used to provide prognostic information, but this assay is not available in most laboratories. The discovery of the expression of the protein tyrosine kinase zeta-associated protein (ZAP)-70 in V(H)-unmutated CLL cases led to its proposal as a surrogate marker for V(H) status. This study investigated the measurement of ZAP-70 expression in CLL using different flow cytometric protocols. Two different antibodies and two different staining methods were compared. The Caltag ZAP-70 antibody and Fix & Perm kit were the easiest to use and were the most sensitive and specific combination, with 91% concordance between ZAP-70 expression and V(H) status. Three patients (9%) were discordant (two V(H) mutated/ZAP-70 positive, and one V(H) unmutated/ZAP-70 negative). No correlation existed between CD38 and either ZAP-70 expression or V(H) status. Measurement of ZAP-70 expression using the Caltag antibody/kit combination provides a standardized flow cytometric method that could be introduced into a routine CLL immunophenotyping panel in a clinical diagnostic laboratory.
Authors:
G Gibbs; T Bromidge; D Howe; J Hopkins; S Johnson
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical and laboratory haematology     Volume:  27     ISSN:  0141-9854     ISO Abbreviation:  Clin Lab Haematol     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-28     Completed Date:  2005-11-08     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  7907061     Medline TA:  Clin Lab Haematol     Country:  England    
Other Details:
Languages:  eng     Pagination:  258-66     Citation Subset:  IM    
Affiliation:
Department of Haematology, Taunton & Somerset Hospital, Taunton TA1 5DA, Somerset, UK. graham.gibbs@tst.nhs.uk
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Clinical Laboratory Techniques*
Female
Flow Cytometry / methods*
Gene Expression Regulation, Neoplastic
Humans
Immunoglobulin Heavy Chains / genetics
Immunoglobulin Variable Region / genetics
Leukemia, Lymphocytic, Chronic, B-Cell / diagnosis*,  genetics*
Male
Middle Aged
Protein-Tyrosine Kinases / analysis*,  genetics*
Reagent Kits, Diagnostic
Sensitivity and Specificity
ZAP-70 Protein-Tyrosine Kinase
Chemical
Reg. No./Substance:
0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Variable Region; 0/Reagent Kits, Diagnostic; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.2/ZAP-70 Protein-Tyrosine Kinase; EC 2.7.10.2/ZAP70 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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