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Comparison of five methods for oxacillin susceptibility testing of Staphylococcus aureus isolates from cystic fibrosis patients.
MedLine Citation:
PMID:  23152311     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Methicillin-resistant Staphylococcus aureus (MRSA) are now a worldwide problem. Cystic fibrosis (CF) patients are commonly colonized and infected by MRSA. Accurate oxacillin susceptibility testing is mandatory for the adequate management of these patients. We performed a comparison of the accuracy of different tests in CF isolates, including methicillin-susceptible S. aureus and MRSA with different SCCmec types, and using the mecA gene as the gold-standard. The sensitivity and specificity of oxacillin disc, Etest, and oxacillin agar screening plate were 100%. Sensitivity of the cefoxitin disc was 85% and specificity was 100%. For clinically relevant isolates, laboratories may consider the use of a combination of two phenotypic methods.
Authors:
Marcelo J Mimica; Rozane L Carvalho; Eitan N Berezin; Neiva Damaceno; Hélio H Caiaffa-Filho
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Revista do Instituto de Medicina Tropical de São Paulo     Volume:  54     ISSN:  1678-9946     ISO Abbreviation:  Rev. Inst. Med. Trop. Sao Paulo     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7507484     Medline TA:  Rev Inst Med Trop Sao Paulo     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  305-6     Citation Subset:  IM    
Affiliation:
Division of Microbiology, Department of Pathology, Santa Casa School of Medicine, São Paulo, SP, Brazil.
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