Document Detail

Comparison of fertility between intracytoplasmic sperm injection and in vitro fertilization with a partial zona pellucida incision by using a piezo-micromanipulator in cryopreserved inbred mouse spermatozoa.
MedLine Citation:
PMID:  14984285     Owner:  NLM     Status:  MEDLINE    
Cryopreservation of mouse spermatozoa has been widely applied for maintenance of transgenic and knockout lines. However, the fertility of cryopreserved spermatozoa from some inbred strains such as C57BL/6 and BALB/c is extremely poor. We have recently reported that a partial zona-pellucida incision by piezo-micromanipulator (ZIP) significantly improves the fertilization rate and subsequent embryonic development after in vitro fertilization (IVF) using cryopreserved C57BL/6 transgenic mouse spermatozoa and that inbred C57BL/6 mice could be produced by intracytoplasmic sperm injection (ICSI). These findings prompted us to compare the efficiency of fertilization and subsequent embryonic development between ICSI and IVF with ZIP (ZIP/IVF) using cryopreserved spermatozoa. In conventional IVF, BALB/cA, C57BL/6J, and B6C3F1 cryopreserved spermatozoa fertilized 19%, 0%, and 51% of oocytes, respectively. The fertilization rates of manipulated oocytes by ICSI versus ZIP/IVF using cryopreserved BALB/cA spermatozoa were 52% versus 68%, cryopreserved C57BL/6J spermatozoa were 43% versus 63%, and cryopreserved B6C3F1 spermatozoa were 58% versus 82%, respectively. In these strains, fertilization rates for ZIP/IVF were significantly higher (P < 0.05) than for other techniques. However, embryonic development to term for oocytes fertilized by cryopreserved spermatozoa was not significantly different between ZIP/IVF and ICSI in C57BL/6J and B6C3F1. The overall efficiency of mouse production in ZIP/IVF was higher than for ICSI and conventional IVF in C57BL/6J and B6C3F1. Furthermore, ZIP/IVF required approximately 3.3 times less manipulation time than did ICSI. Our results indicate that ZIP is a useful assisted reproductive technique for IVF of ova by cryopreserved spermatozoa and improves production in some mouse strains.
Yosuke Kawase; Yukari Aoki; Nobuo Kamada; Kou-Ichi Jishage; Hiroshi Suzuki
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science     Volume:  43     ISSN:  1060-0558     ISO Abbreviation:  Contemp Top Lab Anim Sci     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-02-26     Completed Date:  2004-03-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9204153     Medline TA:  Contemp Top Lab Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  21-5     Citation Subset:  IM    
Chugai Institute for Medical Science, Inc., Japan.
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MeSH Terms
Cryopreservation / veterinary*
Fertility / physiology*
Fertilization in Vitro / methods,  veterinary*
Micromanipulation / methods,  veterinary*
Oocytes / physiology
Sperm Injections, Intracytoplasmic / methods,  veterinary*
Spermatozoa / physiology*
Zona Pellucida

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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