| Comparison of fertility between intracytoplasmic sperm injection and in vitro fertilization with a partial zona pellucida incision by using a piezo-micromanipulator in cryopreserved inbred mouse spermatozoa. | |
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MedLine Citation:
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PMID: 14984285 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cryopreservation of mouse spermatozoa has been widely applied for maintenance of transgenic and knockout lines. However, the fertility of cryopreserved spermatozoa from some inbred strains such as C57BL/6 and BALB/c is extremely poor. We have recently reported that a partial zona-pellucida incision by piezo-micromanipulator (ZIP) significantly improves the fertilization rate and subsequent embryonic development after in vitro fertilization (IVF) using cryopreserved C57BL/6 transgenic mouse spermatozoa and that inbred C57BL/6 mice could be produced by intracytoplasmic sperm injection (ICSI). These findings prompted us to compare the efficiency of fertilization and subsequent embryonic development between ICSI and IVF with ZIP (ZIP/IVF) using cryopreserved spermatozoa. In conventional IVF, BALB/cA, C57BL/6J, and B6C3F1 cryopreserved spermatozoa fertilized 19%, 0%, and 51% of oocytes, respectively. The fertilization rates of manipulated oocytes by ICSI versus ZIP/IVF using cryopreserved BALB/cA spermatozoa were 52% versus 68%, cryopreserved C57BL/6J spermatozoa were 43% versus 63%, and cryopreserved B6C3F1 spermatozoa were 58% versus 82%, respectively. In these strains, fertilization rates for ZIP/IVF were significantly higher (P < 0.05) than for other techniques. However, embryonic development to term for oocytes fertilized by cryopreserved spermatozoa was not significantly different between ZIP/IVF and ICSI in C57BL/6J and B6C3F1. The overall efficiency of mouse production in ZIP/IVF was higher than for ICSI and conventional IVF in C57BL/6J and B6C3F1. Furthermore, ZIP/IVF required approximately 3.3 times less manipulation time than did ICSI. Our results indicate that ZIP is a useful assisted reproductive technique for IVF of ova by cryopreserved spermatozoa and improves production in some mouse strains. |
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Authors:
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Yosuke Kawase; Yukari Aoki; Nobuo Kamada; Kou-Ichi Jishage; Hiroshi Suzuki |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science Volume: 43 ISSN: 1060-0558 ISO Abbreviation: Contemp Top Lab Anim Sci Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-02-26 Completed Date: 2004-03-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9204153 Medline TA: Contemp Top Lab Anim Sci Country: United States |
Other Details:
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Languages: eng Pagination: 21-5 Citation Subset: IM |
Affiliation:
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Chugai Institute for Medical Science, Inc., Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cryopreservation / veterinary* Female Fertility / physiology* Fertilization in Vitro / methods, veterinary* Male Mice Micromanipulation / methods, veterinary* Oocytes / physiology Sperm Injections, Intracytoplasmic / methods, veterinary* Spermatozoa / physiology* Zona Pellucida |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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