| Comparison of the fecal microbiota profiles between ulcerative colitis and Crohn's disease using terminal restriction fragment length polymorphism analysis. | |
| | |
MedLine Citation:
|
PMID: 21253779 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND: Terminal restriction fragment length polymorphism (T-RFLP) analysis is a powerful tool to assess the diversity of a microbial community. In this study, we performed T-RFLP analysis of the fecal microbiota from patients with ulcerative colitis (UC) and those with Crohn's disease (CD). METHODS: Thirty-one patients with UC, 31 patients with CD, and 30 healthy individuals were enrolled. The polymerase chain reaction (PCR) products obtained from the 16S rRNA genes of fecal samples were digested with BslI, and T-RF lengths were determined. RESULTS: The fecal microbial communities were classified into 5 clusters. Twenty-eight of the 30 healthy individuals and 17 of the 18 patients with inactive UC were classified into clusters I, II, and III, but these clusters included a small number of patients with active UC and inactive/active CD. In contrast, 8 of the 13 patients with active UC and the majority of CD patients (12 of the 16 patients with inactive CD, and 11 of the 15 patients with active CD) were included in clusters IV and V. Based on the BslI-digested T-RFLP database, the bacteria showed a significant decrease in the Clostridium family in patients with active UC and inactive/active CD. In contrast, Bacteroides were significantly increased in CD patients. No significant differences were observed between patients with active UC and those with active CD. CONCLUSION: The fecal microbial communities of IBD patients were different from those of healthy individuals. The gut microbiota of patients with inactive UC tended to be closer to that of healthy individuals, suggesting different roles for the fecal microbiota in the pathophysiology of UC and CD. |
| | |
Authors:
|
Akira Andoh; Hirotsugu Imaeda; Tomoki Aomatsu; Osamu Inatomi; Shigeki Bamba; Masaya Sasaki; Yasuharu Saito; Tomoyuki Tsujikawa; Yoshihide Fujiyama |
Related Documents
:
|
21206379 - Association of mycobacterium avium subspecies paratuberculosis with crohn disease in pe... 3302199 - Portal vein dynamics in biliary atresia. 11206869 - Intrapulmonary vascular dilatation in portal hypertension due to cirrhosis of liver. 8741709 - Evaluation of interferon treatment in cirrhotic patients with hepatitis c. 6258829 - Alpha 1-antitrypsin in patients with hepatocellular carcinoma and chronic active hepati... 178399 - Plasma cyclic adenosine-3', 5'-monophosphate response to glucagon in patients with live... 10341019 - Bone mineral density of patients with thalassemia major: four-year follow-up. 2621769 - Silent myocardial ischemia during holter monitoring in ischemic heart disease. 3188829 - The influence of position on ventilation-perfusion distribution after abdominal surgery. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-1-21 |
Journal Detail:
|
Title: Journal of gastroenterology Volume: - ISSN: 1435-5922 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2011-1-21 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9430794 Medline TA: J Gastroenterol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Division of Mucosal Immunology, Graduate School of Medicine, Shiga University of Medical Science, Seta Tsukinowa, Otsu, 520-2192, Japan, andoh@belle.shiga-med.ac.jp. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Postoperative complications and survival rates for pancreatic cancer patients.
Next Document: Computer tomography assessment of pedicle screw placement in lumbar and sacral spine: comparison bet...