Document Detail


Comparison of fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods.
MedLine Citation:
PMID:  11916678     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A 24-h direct plating method for fecal coliform enumeration with a resuscitation step (preincubation for 2 h at 37 +/- 1 degrees C and transfer to 44 +/- 1 degrees C for 22 h) using fecal coliform agar (FCA) was compared with the 24-h standardized violet red bile lactose agar (VRBL) method. FCA and VRBL have equivalent specificities and sensitivities, except for lactose-positive non-fecal coliforms such as Hafnia alvei, which could form typical colonies on FCA and VRBL. Recovery of cold-stressed Escherichia coli in mashed potatoes on FCA was about 1 log unit lower than that with VRBL. When the FCA method was compared with standard VRBL for enumeration of fecal coliforms, based on counting carried out on 170 different food samples, results were not significantly different (P > 0.05). Based on 203 typical identified colonies selected as found on VRBL and FCA, the latter medium appears to allow the enumeration of more true fecal coliforms and has higher performance in certain ways (specificity, sensitivity, and negative and positive predictive values) than VRBL. Most colonies clearly identified on both media were E. coli and H. alvei, a non-fecal coliform. Therefore, the replacement of fecal coliform enumeration by E. coli enumeration to estimate food sanitary quality should be recommended.
Authors:
A Leclercq; C Wanegue; P Baylac
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-03-27     Completed Date:  2002-07-12     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1631-8     Citation Subset:  IM    
Affiliation:
Pôle Santé-Aliment-Nutrition, Institut Pasteur de Lille, Domaine du Certia, F-59651 Villeneuve d'Ascq, France. Alexandre.Leclercq@pasteur-lille.fr
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MeSH Terms
Descriptor/Qualifier:
Agar
Animals
Bacteriological Techniques
Colony Count, Microbial
Culture Media
Enterobacteriaceae / isolation & purification*
Escherichia coli / isolation & purification
Feces / microbiology*
Food Microbiology*
Hafnia alvei / isolation & purification
Humans
Sensitivity and Specificity
Yeasts / isolation & purification
Chemical
Reg. No./Substance:
0/Culture Media; 9002-18-0/Agar
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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