Document Detail

Comparison of fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods.
MedLine Citation:
PMID:  11916678     Owner:  NLM     Status:  MEDLINE    
A 24-h direct plating method for fecal coliform enumeration with a resuscitation step (preincubation for 2 h at 37 +/- 1 degrees C and transfer to 44 +/- 1 degrees C for 22 h) using fecal coliform agar (FCA) was compared with the 24-h standardized violet red bile lactose agar (VRBL) method. FCA and VRBL have equivalent specificities and sensitivities, except for lactose-positive non-fecal coliforms such as Hafnia alvei, which could form typical colonies on FCA and VRBL. Recovery of cold-stressed Escherichia coli in mashed potatoes on FCA was about 1 log unit lower than that with VRBL. When the FCA method was compared with standard VRBL for enumeration of fecal coliforms, based on counting carried out on 170 different food samples, results were not significantly different (P > 0.05). Based on 203 typical identified colonies selected as found on VRBL and FCA, the latter medium appears to allow the enumeration of more true fecal coliforms and has higher performance in certain ways (specificity, sensitivity, and negative and positive predictive values) than VRBL. Most colonies clearly identified on both media were E. coli and H. alvei, a non-fecal coliform. Therefore, the replacement of fecal coliform enumeration by E. coli enumeration to estimate food sanitary quality should be recommended.
A Leclercq; C Wanegue; P Baylac
Related Documents :
22063718 - Cannabinoid facilitation of behavioral and biochemical hedonic taste responses.
19272008 - A scorpion probe-based real-time pcr assay for detection of e. coli o157:h7 in dairy pr...
10446288 - The ion coupling and organic substrate specificities of osmoregulatory transporter prop...
3722028 - The enumeration of thermotrophic types amongst the enterobacteriaceae colonizing perish...
9779608 - Killer activity of tilletiopsis albescens gokhale: taxonomic and phylogenetic implication.
18429698 - Regulation of food intake through hypothalamic signaling networks involving mtor.
Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-03-27     Completed Date:  2002-07-12     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1631-8     Citation Subset:  IM    
Pôle Santé-Aliment-Nutrition, Institut Pasteur de Lille, Domaine du Certia, F-59651 Villeneuve d'Ascq, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacteriological Techniques
Colony Count, Microbial
Culture Media
Enterobacteriaceae / isolation & purification*
Escherichia coli / isolation & purification
Feces / microbiology*
Food Microbiology*
Hafnia alvei / isolation & purification
Sensitivity and Specificity
Yeasts / isolation & purification
Reg. No./Substance:
0/Culture Media; 9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The ybiT gene of Erwinia chrysanthemi codes for a putative ABC transporter and is involved in compet...
Next Document:  Purification and characterization of thermostable endo-1,5-alpha-L-arabinase from a strain of Bacill...