Document Detail

Comparison of the expression of cell surface poly-N-acetyllactosamine-type oligosaccharides in PC12 cells with those in its variant PC12D.
MedLine Citation:
PMID:  11445553     Owner:  NLM     Status:  MEDLINE    
To explore the biological role of carbohydrate chains in the process of nerve cell differentiation, we carried out a characterization of the carbohydrate structure of glycoproteins by comparing conventional PC12 cells with variant cells (PC12D). In vitro metabolic labeling of cells with either [(3)H] glucosamine or [(3)H] threonine, together with tomato lectin staining, revealed that nerve growth factor (NGF) stimulation caused a decrease in the poly-N-acetyllactosamine synthesis of high-molecular-weight glycopeptides from PC12 cells. By comparison, the amount of glycopeptides with poly-N-acetyllactosamine from PC12D cells was already significantly low and it was not changed by NGF stimulation. By assaying the glycosyltransferases that participate in poly-N-acetyllactosamine synthesis, the decrease in the amount of the poly-N-acetyllactosamine in PC12D cells as well as NGF-stimulated PC12 cells could be accounted for by a reduction in the activity of poly-N-acetyllactosamine extension enzyme (GnT-i), because the amount of poly-N-acetyllactosamine in both cells precisely correlated with changes in GnT-i activity, whereas the activities of N-acetylglucosaminyltransferase V (GnT-V) and beta 1-4 galactosyltransferase remained unchanged. These results demonstrate that the decrease in poly-N-acetyllactosamine synthesis in PC12 cells occurred prior to neurite formation, whereas PC12D cells were insensitive to this effect. Next, we showed that GnT-i but not GnT-V catalyzed a rate-limiting reaction in the expression of poly-N-acetyllactosamine chains, especially in pheochromocytoma.
M Fukuzumi; S Maruyama; M Sano; S Fukui
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycobiology     Volume:  11     ISSN:  0959-6658     ISO Abbreviation:  Glycobiology     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-07-10     Completed Date:  2001-10-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9104124     Medline TA:  Glycobiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  481-94     Citation Subset:  IM    
Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, Kita-ku, Kyoto 603-8555, Japan.
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MeSH Terms
Carbohydrate Sequence
Cell Differentiation
Glucosamine / metabolism
Membrane Glycoproteins / biosynthesis*
Molecular Sequence Data
N-Acetylglucosaminyltransferases / metabolism
Nerve Growth Factor / pharmacology
Oligosaccharides / biosynthesis*
PC12 Cells / cytology,  drug effects,  metabolism*
Polysaccharides / biosynthesis*
Reg. No./Substance:
0/Membrane Glycoproteins; 0/Oligosaccharides; 0/Polysaccharides; 3416-24-8/Glucosamine; 82441-98-3/poly-N-acetyllactosamine; 9061-61-4/Nerve Growth Factor; EC 2.4.1.-/N-Acetylglucosaminyltransferases; EC,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I

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