| Comparison of the expression of cell surface poly-N-acetyllactosamine-type oligosaccharides in PC12 cells with those in its variant PC12D. | |
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MedLine Citation:
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PMID: 11445553 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To explore the biological role of carbohydrate chains in the process of nerve cell differentiation, we carried out a characterization of the carbohydrate structure of glycoproteins by comparing conventional PC12 cells with variant cells (PC12D). In vitro metabolic labeling of cells with either [(3)H] glucosamine or [(3)H] threonine, together with tomato lectin staining, revealed that nerve growth factor (NGF) stimulation caused a decrease in the poly-N-acetyllactosamine synthesis of high-molecular-weight glycopeptides from PC12 cells. By comparison, the amount of glycopeptides with poly-N-acetyllactosamine from PC12D cells was already significantly low and it was not changed by NGF stimulation. By assaying the glycosyltransferases that participate in poly-N-acetyllactosamine synthesis, the decrease in the amount of the poly-N-acetyllactosamine in PC12D cells as well as NGF-stimulated PC12 cells could be accounted for by a reduction in the activity of poly-N-acetyllactosamine extension enzyme (GnT-i), because the amount of poly-N-acetyllactosamine in both cells precisely correlated with changes in GnT-i activity, whereas the activities of N-acetylglucosaminyltransferase V (GnT-V) and beta 1-4 galactosyltransferase remained unchanged. These results demonstrate that the decrease in poly-N-acetyllactosamine synthesis in PC12 cells occurred prior to neurite formation, whereas PC12D cells were insensitive to this effect. Next, we showed that GnT-i but not GnT-V catalyzed a rate-limiting reaction in the expression of poly-N-acetyllactosamine chains, especially in pheochromocytoma. |
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Authors:
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M Fukuzumi; S Maruyama; M Sano; S Fukui |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Glycobiology Volume: 11 ISSN: 0959-6658 ISO Abbreviation: Glycobiology Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-07-10 Completed Date: 2001-10-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9104124 Medline TA: Glycobiology Country: England |
Other Details:
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Languages: eng Pagination: 481-94 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, Kita-ku, Kyoto 603-8555, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrate Sequence Cell Differentiation Glucosamine / metabolism Membrane Glycoproteins / biosynthesis* Molecular Sequence Data N-Acetylglucosaminyltransferases / metabolism Nerve Growth Factor / pharmacology Neurites* Oligosaccharides / biosynthesis* PC12 Cells / cytology, drug effects, metabolism* Polysaccharides / biosynthesis* Rats |
| Chemical | |
Reg. No./Substance:
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0/Membrane Glycoproteins; 0/Oligosaccharides; 0/Polysaccharides; 3416-24-8/Glucosamine; 82441-98-3/poly-N-acetyllactosamine; 9061-61-4/Nerve Growth Factor; EC 2.4.1.-/N-Acetylglucosaminyltransferases; EC 2.4.1.101/alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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