Document Detail


Comparison of enzyme-linked immunosorbent assay with dot immunobinding assay for detection of antibodies against Pasteurella multocida in turkeys.
MedLine Citation:
PMID:  2241679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A dot immunobinding assay (DIA) was developed to detect antibodies against Pasteurella multocida in turkey serum. Five coating antigens, namely, whole-cell (WC) antigen, sonicated cell lysate (SCL), crude capsular extract (CE), formalin extract (FE), and heat-stable antigen (HSA), were compared by enzyme-linked immunosorbent assay (ELISA) and DIA using reference antisera against P. multocida organisms. WC and SCL antigens showed higher sensitivity, whereas FE and HSA antigens were more specific coating antigens in both assays. The specificity of DIA was greater than ELISA by comparing the P/N ratios of HSA against serum prepared from heterologous serotype of P. multocida. The DIA had also several distinct advantages over the ELISA, which included reduction of the manipulation time and more uniform binding of coating antigens onto the nitrocellulose membranes compared with binding of coating antigens to microtiter plates for ELISA.
Authors:
K H Choi; S K Maheswaran; T W Molitor
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Avian diseases     Volume:  34     ISSN:  0005-2086     ISO Abbreviation:  Avian Dis.     Publication Date:    1990 Jul-Sep
Date Detail:
Created Date:  1990-12-07     Completed Date:  1990-12-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370617     Medline TA:  Avian Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  539-47     Citation Subset:  IM    
Affiliation:
Department of Veterinary Pathobiology, University of Minnesota, St. Paul 55108.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Bacterial / analysis*
Enzyme-Linked Immunosorbent Assay
Immunoblotting
Pasteurella / immunology*
Pasteurella Infections / immunology,  veterinary*
Poultry Diseases / immunology*
Predictive Value of Tests
Turkeys*
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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