Document Detail


Comparison of the effects of vasopressin and norepinephrine on organ perfusion during septic shock in streptozotocin-induced diabetic rats.
MedLine Citation:
PMID:  20390307     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The purpose of this study was to compare the effects of norepinephrine (NE) and vasopressin on systemic hemodynamics, renal and mesenteric artery blood flow, inflammatory response and inducible nitric oxide synthase (iNOS) activity during endotoxin shock in streptozotocin-induced diabetic rats.
METHODS: The study was designed to include three sets of experiments: (1) measurement of changes in systemic hemodynamics and mesenteric and renal artery blood flow; (2) measurement of biochemical variables; and (3) measurement of iNOS activity in the mesenteric artery. Systemic hemodynamics, regional artery blood flow changes and biochemical variables were assessed before treatment and 1, 2 and 3 h after treatment.
RESULTS: Vasopressin, but not NE, prevented the decreases in aortic blood flow, but did not restore mesenteric artery blood flow. In addition, vasopressin partially restored renal artery blood flow in diabetic rats. Plasma nitrite levels and iNOS activity in the mesenteric artery were elevated after intravenous LPS in diabetic rats. Endotoxin-induced decreases in mesenteric arterial blood flow were partially restored by vasopressin with nonselective NOS inhibitor, N G-nitro-l-arginine methyl ester (l-NAME), in diabetic rats. Moreover, l-NAME prevented increases in plasma nitrite levels and iNOS activity in the mesenteric artery. In contrast, endotoxin-induced decreases in renal arterial blood flow were partially restored by vasopressin with l-NAME, but not by NE in diabetic rats.
CONCLUSIONS: Nitric oxide may be one possible contributor to reduced sensitivity of the mesenteric and renal arteries to vasopressin during septic shock in streptozotocin-induced diabetic rats.
Authors:
Hiroshi Hinohara; Yuji Kadoi; Aya Tokue; Shigeru Saito; Chikara Kawauchi; Akio Mizutani
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of anesthesia     Volume:  24     ISSN:  1438-8359     ISO Abbreviation:  J Anesth     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-16     Completed Date:  2010-12-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8905667     Medline TA:  J Anesth     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  553-63     Citation Subset:  IM    
Affiliation:
Department of Anesthesiology, Graduate School of Medicine, Gunma University, 3-39-22 Showa-Machi, Maebashi, Gunma 371-8511, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Diabetes Mellitus, Experimental / physiopathology*
Hemodynamics / drug effects*
Male
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide / physiology
Norepinephrine / pharmacology*
Rats
Rats, Wistar
Renal Circulation / drug effects
Shock, Septic / drug therapy*,  physiopathology
Splanchnic Circulation / drug effects
Streptozocin
Vasopressins / pharmacology*
Chemical
Reg. No./Substance:
10102-43-9/Nitric Oxide; 11000-17-2/Vasopressins; 18883-66-4/Streptozocin; 50903-99-6/NG-Nitroarginine Methyl Ester; 51-41-2/Norepinephrine

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