Document Detail

Comparison of effects of two hemoglobin-based O(2) carriers on intestinal integrity and microvascular leakage.
MedLine Citation:
PMID:  12234778     Owner:  NLM     Status:  MEDLINE    
Two "blood substitutes," a diaspirin cross-linked human hemoglobin [bis(3,5 dibromosalicyl)fumarate, DBBF-Hb] and a bovine polymerized hemoglobin (PolyHbBv), advanced to clinical trials, are used in this study. Previously, we have shown that injection of DBBF-Hb into the rat circulation produces venular leakage and intestinal epithelial disruption. The purpose of this study was to determine whether PolyHbBv, currently approved for veterinary use in the United States, shows similar effects. In anesthetized Sprague-Dawley rats, the mesenteric microvasculature was perfused with DBBF-Hb (n = 6), PolyHbBv (n = 5), cyanomet Hb (CNmet-DBBF-Hb), or HEPES-buffered saline with 0.5% bovine serum albumin (HBS-BSA) (controls, n = 7) for 10 min, followed by FITC-albumin for 3 min, and then fixed for microscopy. For DBBF-Hb, the mean leak number per micrometer venule length [2.41 +/- 0.33 (+/-SE) x 10(-3)] was significantly greater than for PolyHbBv (0.53 +/- 0.14 x 10(-3)), CNmet-DBBF-Hb (0.36 +/- 0.14 x 10(-3)), and HBS-BSA (0.12 +/- 0.08 x 10(-3)) (P < 0.01). Corresponding quantities for leak area were 0.10 +/- 0.03, 0.010 +/- 0.003, 0.005 +/- 0.003, and 0.02 +/- 0.02 microm(2)/microm. In rats injected with DBBF-Hb (n = 8), intestinal epithelial integrity was significantly compromised compared with those injected with PolyHbBv (n = 5) or saline (n = 6). These results indicate that intravascular PolyHbBv produces significantly less disruption of the intestinal exchange barrier than does DBBF-Hb, probably because the heme is not so easily oxidized.
Ann L Baldwin; Elizabeth B Wiley; Abdu I Alayash
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  283     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-09-17     Completed Date:  2002-10-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1292-301     Citation Subset:  IM    
Department of Physiology, College of Medicine, University of Arizona, Tucson 85724-5051, USA.
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MeSH Terms
Aspirin / analogs & derivatives*,  pharmacology*
Blood Substitutes / pharmacology
Cell Degranulation / drug effects
Goblet Cells / secretion
Hemoglobins / pharmacology*
Intestinal Mucosa / blood supply*,  drug effects*,  pathology
Mast Cells / metabolism
Microcirculation / drug effects
Microscopy, Electron
Microscopy, Fluorescence
Rats, Sprague-Dawley
Reactive Oxygen Species / metabolism
Splanchnic Circulation / drug effects
Venules / drug effects,  metabolism
Grant Support
Reg. No./Substance:
0/Blood Substitutes; 0/Hemoglobins; 0/Reactive Oxygen Species; 0/bis(3,5-dibromosalicyl)sebacate-crosslinked hemoglobin; 0/polyhemoglobin; 50-78-2/Aspirin

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