| Comparison of the effects of ozone on the modification of amino acid residues in glutamine synthetase and bovine serum albumin. | |
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MedLine Citation:
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PMID: 8626759 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During exposure to ozone, the methionine and aromatic amino acid residues of Escherichia coli glutamine synthetase (GS) and bovine serum albumin (BSA) are oxidized rapidly in the order Met > Trp > Tyr approximately His > Phe. The loss of His is matched by a nearly equivalent formation of aspartate or of a derivative that is converted to aspartic acid upon acid hydrolysis. Conversion of His to aspartate was confirmed by showing that the oxidation of E. coli protein in which all His residues were uniformly labeled with 14C gave rise to 14C-labeled aspartic acid in 80% yield and also by the demonstration that His residues in the tripeptides Ala-His-Ala or Ala-Ala-His gave rise to nearly stoichiometric amounts of aspartic acid whereas oxidation of His-Ala-Ala yielded only 36% aspartate. The oxidation of BSA and GS led to formation, respectively, of 11 and 3.3 eq of carbonyl groups and 0.5 and 0.3 eq of quinoprotein per subunit. Although BSA and GS contain nearly identical amounts of each kind of aromatic amino acid residues, oxidation of these residues in BSA was about 1.5-2.0 times faster than in GS indicating that the susceptibility to oxidation is dependent on the primary, secondary, tertiary, and quaternary structure of the protein. |
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Authors:
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B S Berlett; R L Levine; E R Stadtman |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 271 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1996-06-21 Completed Date: 1996-06-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4177-82 Citation Subset: IM |
Affiliation:
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Laboratory of Biochemistry, NHLBI, National Institutes of Health, Bethesda, Maryland 20892-0342, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids* Animals Cattle Escherichia coli / enzymology Glutamate-Ammonia Ligase / chemistry, drug effects*, metabolism Histidine Kinetics Methionine* Molecular Sequence Data Oligopeptides / chemistry Oxidation-Reduction Ozone / pharmacology* Phenylalanine Serum Albumin, Bovine / chemistry, drug effects*, metabolism Structure-Activity Relationship Tryptophan Tyrosine |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Oligopeptides; 0/Serum Albumin, Bovine; 10028-15-6/Ozone; 55520-40-6/Tyrosine; 63-68-3/Methionine; 63-91-2/Phenylalanine; 71-00-1/Histidine; 73-22-3/Tryptophan; EC 6.3.1.2/Glutamate-Ammonia Ligase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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