Document Detail


Comparison of the effects of immunosuppressive factors from newly established colon carcinoma cell cultures on human lymphocyte proliferation and cytokine secretion.
MedLine Citation:
PMID:  10601837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tumor cells may influence the host's immune reactivity by the production of immunosuppressive factors (ISFs). In this study, the effects of ISFs derived from nine polyclonal colorectal carcinoma (CRC) cell lines on PHA-induced lymphocyte proliferation and cytokine secretion was investigated. We found that most of the culture supernatants (8/9) from CRC cell lines contained ISFs, which inhibited T cell proliferation to a variable degree in a dose-dependent manner. Comparison of T cell proliferation in the presence or absence of monocytes showed that monocytes can modulate the effects of tumor-derived ISFs on lymphocyte function. In addition, exposure of activated PBMC to the tumor cell supernatants resulted in an altered secretion of cytokines by these cells, i.e. the secretion of IFN-gamma was generally reduced while the secretion of IL-1beta, IL-2 and TNF-alpha was little affected. We further investigated the supernatants' inhibitory effects on PBMC in respect to the production of prostaglandin E(2) (PGE(2)). It was found that PGE(2) was secreted by all tumor cell cultures. Therefore this substance is probably involved in the immunosuppression in vivo. However, the secreted PGE(2) was shown not to be solely responsible for the observed suppression of lymphocyte proliferation in vitro. Our results suggest that the secretion of ISF is a common property of CRCs as demonstrated with newly established CRC cell cultures, and therefore this may also be an important immune escape mechanism of colonic carcinomas in vivo.
Authors:
J S Luo; R Kammerer; S von Kleist
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine     Volume:  21     ISSN:  1010-4283     ISO Abbreviation:  Tumour Biol.     Publication Date:    2000 Jan-Feb
Date Detail:
Created Date:  2000-02-18     Completed Date:  2000-02-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8409922     Medline TA:  Tumour Biol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  11-20     Citation Subset:  IM    
Copyright Information:
Copyright 2000 S. Karger AG, Basel
Affiliation:
Institute of Immunobiology, University of Freiburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques
Cell Division / immunology
Cell Survival / immunology
Cell-Free System / immunology
Colonic Neoplasms / immunology*,  pathology,  secretion*
Cytokines / biosynthesis,  secretion*
Dinoprostone / biosynthesis,  physiology
Growth Inhibitors / physiology
HT29 Cells
Humans
Leukocytes, Mononuclear / metabolism
Lymphocyte Activation / immunology*
Monocytes / immunology
Neoplasm Proteins / physiology*
Suppressor Factors, Immunologic / physiology*
T-Lymphocytes / cytology,  immunology,  metabolism
Chemical
Reg. No./Substance:
0/Cytokines; 0/Growth Inhibitors; 0/Neoplasm Proteins; 0/Suppressor Factors, Immunologic; 363-24-6/Dinoprostone

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