Document Detail

Comparison of effects of PKA catalytic subunit on I(h) and calcium channel currents in rat dorsal root ganglion cells.
MedLine Citation:
PMID:  17878598     Owner:  NLM     Status:  MEDLINE    
We investigated whether PKA-induced phosphorylation was involved in regulation of hyperpolarization-activated current (I(h)) in rat dorsal root ganglion (DRG) cells. We examined the effect of the catalytic subunit of PKA (PKAc) on I(h) and confirmed an effect of PKAc on Ca(2+) channel currents carried by Ba(2+) (I(Ba)) in identical neurons as a positive control of PKA activity. After the start of recording, amplitudes of I(Ba) gradually decreased (rundown). An intracellular application of ATP reduced the rundown of I(Ba) and induced a depolarizing shift of I(h) activation. The former was partially reversed by PKI but the latter was not affected. An intracellular application of PKAc also prevented the rundown of I(Ba) and this effect was potentiated by okadaic acid (OA). The application of PKAc and OA in combination did not change the electrophysiological properties of I(h) although a potentiating effect on I(Ba) was observed in the same neurons. The application of 2-mM ATP in addition to PKAc and OA did not result in an additional potentiation of I(Ba), but shifted the activation curve of I(h) positively. These results suggested that PKA-induced phosphorylation was not involved in the modulatory mechanisms of I(h) in rat DRG neurons.
You Komagiri; Naoki Kitamura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical research (Tokyo, Japan)     Volume:  28     ISSN:  0388-6107     ISO Abbreviation:  Biomed. Res.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-09-19     Completed Date:  2007-10-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8100317     Medline TA:  Biomed Res     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  177-89     Citation Subset:  IM    
Laboratory of Physiology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
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MeSH Terms
Adenosine Triphosphate / pharmacology
Barium / pharmacology
Calcium Channels / metabolism*
Catalytic Domain / physiology
Cyclic AMP-Dependent Protein Kinases
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Ganglia, Spinal / cytology,  enzymology*
Membrane Potentials / drug effects,  physiology*
Nerve Tissue Proteins / metabolism*
Neurons / enzymology*
Okadaic Acid / pharmacology
Protein-Serine-Threonine Kinases / metabolism*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Calcium Channels; 0/Enzyme Inhibitors; 0/Nerve Tissue Proteins; 56-65-5/Adenosine Triphosphate; 7440-39-3/Barium; 78111-17-8/Okadaic Acid; EC Kinases; EC AMP-Dependent Protein Kinases

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